Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3888213..3888907 | Replicon | chromosome |
| Accession | NZ_CP066839 | ||
| Organism | Escherichia coli strain Ec-RL2-1X | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | JH656_RS19180 | Protein ID | WP_001263493.1 |
| Coordinates | 3888213..3888611 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | JH656_RS19185 | Protein ID | WP_000554757.1 |
| Coordinates | 3888614..3888907 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3883873) | 3883873..3883953 | - | 81 | NuclAT_13 | - | - |
| - (3883873) | 3883873..3883953 | - | 81 | NuclAT_13 | - | - |
| - (3883873) | 3883873..3883953 | - | 81 | NuclAT_13 | - | - |
| - (3883873) | 3883873..3883953 | - | 81 | NuclAT_13 | - | - |
| JH656_RS19150 (3883213) | 3883213..3884457 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| JH656_RS19155 (3884549) | 3884549..3885007 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JH656_RS19160 (3885268) | 3885268..3886725 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| JH656_RS19165 (3886782) | 3886782..3887303 | - | 522 | Protein_3754 | peptide chain release factor H | - |
| JH656_RS19170 (3887302) | 3887302..3887505 | - | 204 | Protein_3755 | RtcB family protein | - |
| JH656_RS19175 (3887751) | 3887751..3888203 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| JH656_RS19180 (3888213) | 3888213..3888611 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JH656_RS19185 (3888614) | 3888614..3888907 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JH656_RS19190 (3888959) | 3888959..3890014 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| JH656_RS19195 (3890085) | 3890085..3890870 | - | 786 | WP_000207564.1 | putative lateral flagellar export/assembly protein LafU | - |
| JH656_RS19200 (3890842) | 3890842..3892554 | + | 1713 | Protein_3761 | flagellar biosynthesis protein FlhA | - |
| JH656_RS19205 (3892770) | 3892770..3893267 | - | 498 | WP_000006260.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA / tssC / AHA_1833 / tssF / tssA / hcp1/tssD1 | 3887251..3914562 | 27311 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T185187 WP_001263493.1 NZ_CP066839:c3888611-3888213 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T185187 NZ_CP088092:119275-119763 [Rhizobium laguerreae]
ATGCTGTCTGCACCGACGCTTCTCGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTACGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTCTGCGAGGGCGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTGGCTGTCGCCGACTCGCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCCATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCGATCTCCGATGAAGCCAAGG
CCTTCTATGAGCATTTTGGATTTACATCGTCGCCCGCGAATCCTATGGCCTTGGTGTTATCATTGAAAGCTCAAGTAGGC
CTGAAGTGA
ATGCTGTCTGCACCGACGCTTCTCGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTACGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTCTGCGAGGGCGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTGGCTGTCGCCGACTCGCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCCATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCGATCTCCGATGAAGCCAAGG
CCTTCTATGAGCATTTTGGATTTACATCGTCGCCCGCGAATCCTATGGCCTTGGTGTTATCATTGAAAGCTCAAGTAGGC
CTGAAGTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT185187 WP_000554757.1 NZ_CP066839:c3888907-3888614 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT185187 NZ_CP088092:118991-119275 [Rhizobium laguerreae]
ATGACCAGCAAAAACGCCACTGATACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGTGCCGCCAAGGCCCACGGGAAGACCCGTTCGGATTTCATGATCGACGCTTCGCGCCGTGCCGCAGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAACCGCCAAATAGCGAA
GGCTTCGAACGGCTGATGAACACTCGCAAACCCTGGCAGCCGTAA
ATGACCAGCAAAAACGCCACTGATACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGTGCCGCCAAGGCCCACGGGAAGACCCGTTCGGATTTCATGATCGACGCTTCGCGCCGTGCCGCAGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAACCGCCAAATAGCGAA
GGCTTCGAACGGCTGATGAACACTCGCAAACCCTGGCAGCCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|