Detailed information of TA system TA185173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1013915..1014608
• Replicon: chromosome
• Accession: NZ_CP066839
• Organism: Escherichia coli strain Ec-RL2-1X

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: JH656_RS04970
• Protein ID: WP_000415584.1
• Coordinates: 1013915..1014211 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JH656_RS04975
• Protein ID: WP_000650107.1
• Coordinates: 1014213..1014608 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH656_RS04935 (1009003)	1009003..1009317	-	315	WP_000958598.1	putative quinol monooxygenase	-
JH656_RS04940 (1009348)	1009348..1009929	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JH656_RS04945 (1010248)	1010248..1010580	+	333	WP_000917684.1	DUF2645 family protein	-
JH656_RS04950 (1010626)	1010626..1011975	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
JH656_RS04955 (1011972)	1011972..1012631	-	660	WP_139560537.1	quorum sensing response regulator transcription factor QseB	-
JH656_RS04960 (1012783)	1012783..1013175	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JH656_RS04965 (1013228)	1013228..1013710	+	483	WP_100620835.1	GyrI-like domain-containing protein	-
JH656_RS04970 (1013915)	1013915..1014211	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
JH656_RS04975 (1014213)	1014213..1014608	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JH656_RS04980 (1014741)	1014741..1016348	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
JH656_RS04985 (1016486)	1016486..1018744	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T185173 WP_000415584.1 NZ_CP066839:1013915-1014211 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T185173 NZ_CP088087:3480482-3480742 [Bradyrhizobium arachidis]
GTGAAGATCGTTTGGGACGAACCGAAGCGCCGAGCCAATCTCGACAAGCACGGGATGGATTTCGCTGATCTCAACGAGGC
CTTCTTCGACAATGCCTTGGTTTTGGCCACCTATGGCAACCGCTGGCGCGCGATCGGCATTAACATTCGTGGCGTTGTCT
CAGTCATCTTCGCGACCCGCGGAGCTGAGGGCGTAAGCATCGTCAGCATGCGCCCTGCCAGCAAGAAAGAAAGGAGGCTC
TATGAAGAAAACCGTCTCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT185173 WP_000650107.1 NZ_CP066839:1014213-1014608 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT185173 NZ_CP088087:3480723-3481016 [Bradyrhizobium arachidis]
ATGAAGAAAACCGTCTCTAAGAAGGGTTACACCGCACGGGACCTGCGTGCGGTGAGCGACAATCCGGAATGGACCAAGGA
TGACTTCGCCAAGGCCAAGACTTTCGATGAGGTTTTCCCGGGCATGCGAAAGGGCCGCGGCCCCAATAAAGCCCCCACAA
AGCGACAGGTGACCTTGCGCCTGAGCCCTGTCGTGGTCGACTATTTCAAGGCCGAGGGCCCCGGTTGGCAGAGCCGGATT
GACGAAGCGCTCGTCAAGGTGGTGAAGCGCAAGCGCAGTTCTGCCACGGGTTAG

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65