185172

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 948003..948730 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JH656_RS04655 Protein ID WP_000550189.1
Coordinates 948003..948317 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JH656_RS04660 Protein ID WP_000560266.1
Coordinates 948314..948730 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS04635 (944161) 944161..945147 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
JH656_RS04640 (945226) 945226..945918 - 693 WP_000942546.1 vancomycin high temperature exclusion protein -
JH656_RS04645 (945995) 945995..946498 - 504 WP_001300832.1 M48 family metallopeptidase -
JH656_RS04650 (946583) 946583..947719 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JH656_RS04655 (948003) 948003..948317 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JH656_RS04660 (948314) 948314..948730 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JH656_RS04665 (948775) 948775..950793 - 2019 WP_242561977.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JH656_RS04670 (951219) 951219..953570 - 2352 WP_097296971.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T185172 WP_000550189.1 NZ_CP066839:948003-948317 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T185172 NZ_CP088087:c2674146-2673724 [Bradyrhizobium arachidis]
GTGAACCTGCTGCTCGACACCAACGTACTGTCCGAGGTTCGGCGGCCTGCGCCTTCGCCGAAAGTTCTGGCGTGGCTTGA
TACGATCGATGAGGATCGCACTTTCATCAGTGTTGCCTCGATCGCCGAGCTTCGCCGCGGCGTGGCGTCGCTGGAGGACG
GCCGTCGGCGCACAGCGTTAGCCGCCTGGCTGACTCATGATCTACCGGCGCGGTTTGCCGAACGCGTCCTGCCGATCGAC
CACGCGGTGGCGGAACGCTGGGGCGACCTGATGGCGCAGAGCCGCAGAAGTGGCGTCGTATTGTCTGTCATGGACGGCTT
CTTTGCCGCAACCGCGCTCGCGAACAGCCTCACGCTCGTCACGCGCAATGTGAAGGATTTTGCACCGTTCGGCCTGTCGC
TGCTCAACCCGTGGGACGCCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT185172 WP_000560266.1 NZ_CP066839:948314-948730 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT185172 NZ_CP088087:c2674427-2674143 [Bradyrhizobium arachidis]
ATGGTCGATAGCAACCCTGCACCTGCCACGCTTCCGACTGATGACACTTGGACGCTCGCCAATGCCAAGGCACGGCTGTC
CGAGGTAATCGATCGCGCCCAAACCGGCCCGCAGGTCATCACCCGCCACGGCAAACCCAATGCGGTCGTCGTTTCCGCCG
AGGAATGGGCGCGCAAGGTCGCTCGCAAAGGTACGCTCGCCGAATTCCTGCTGGCGTCGCCGCTGCGTGGCGCAGACCTT
GAGCTCGATCGCGTGCACGACACGCCACGCGACGAAATGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References