Detailed information of TA system TA185168

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50700..50964
• Replicon: plasmid pIncI1_STnovel
• Accession: NZ_CP066838
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: JH476_RS25610
• Protein ID: WP_001387489.1
• Coordinates: 50812..50964 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	50700..50762 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS25595 (46742)	46742..47812	-	1071	WP_016231890.1	IncI1-type conjugal transfer protein TrbB	-
JH476_RS25600 (47831)	47831..49039	-	1209	WP_052978849.1	IncI1-type conjugal transfer protein TrbA	-
JH476_RS25605 (49257)	49257..50210	-	954	WP_016231891.1	hypothetical protein	-
- (50404)	50404..50459	-	56	NuclAT_1	-	-
- (50404)	50404..50459	-	56	NuclAT_1	-	-
- (50404)	50404..50459	-	56	NuclAT_1	-	-
- (50404)	50404..50459	-	56	NuclAT_1	-	-
- (50700)	50700..50762	-	63	NuclAT_0	-	Antitoxin
- (50700)	50700..50762	-	63	NuclAT_0	-	Antitoxin
- (50700)	50700..50762	-	63	NuclAT_0	-	Antitoxin
- (50700)	50700..50762	-	63	NuclAT_0	-	Antitoxin
JH476_RS25610 (50812)	50812..50964	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
JH476_RS25615 (51036)	51036..51287	-	252	WP_001291965.1	hypothetical protein	-
JH476_RS26085 (51649)	51649..51881	+	233	Protein_62	hypothetical protein	-
JH476_RS25620 (51946)	51946..52122	-	177	WP_001054901.1	hypothetical protein	-
JH476_RS25625 (52454)	52454..52663	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
JH476_RS25630 (52735)	52735..53385	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
JH476_RS25635 (53459)	53459..55627	-	2169	WP_016231892.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95616	95616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T185168 WP_001387489.1 NZ_CP066838:50812-50964 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T185168 NZ_CP088082:84166-84870 [Ideonella dechloratans]
ATGGATTGGCAGCCCGACTGGTTCGAGGGGACCATCCGTCCCCAGGCCACGCTGGCCTGGCGCGGAGTGGAGGCACAGCA
CGTCGTCTCCACCATGCGCCTGGTGGACAGCGCCGCCGAACAGGATCTGCTGGAGCAGCTGCTCGAAGGCAGCAAGCCCG
CCCTGCCGCCGGCCGCCCGGCCGCTGCACTACCTGCTGGCCACGCCCTTTCGATACCGGCCGCCGCACGCCAGCCGCTTT
CGCGCGCCGCACAGCCCGGGTCAGTGGTACGGCGCGCAGACGCTGTACGCCGCCTGCGCCGAGGTGGCTTACTGGCGGCA
CCGCTTCATCCTGGACAGCGCCGGCCTGGCCGGGCGCGAGCTGCTGACCGAGCACACCTTCTTCCAGGGCGCCATCCAGG
GTCTGGCCATCGACCTGACCCAGCCGCCCTGGGACCAGGCCCAGGCCCTGTGGACCCACGACAGCGACTACCGTGCGCCC
CAGGCGGTGGCCCGCTCGGCCCAGATCCGCGGCGTGCAGTGGCTGGCCTATGCCTCGGTGCGGGCACCGGGCCACCACTG
CGCCGTGGTCTTCGACCCGGCCAGTCTGGCCGAGCCGCCCGGCGGCCTGGACGCCACCCAGCAGACCTGGCACTGCAAGG
CCACCGCACAGCGGGTCATGTTCGCGCGGGGCGCGGAGCGGTTCGAGTGGGCGTGGACGGGCTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT185168 NZ_CP066838:c50762-50700 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin