Detailed information of TA system TA185164

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101909..102335
• Replicon: plasmid pIncFIB_IncFII
• Accession: NZ_CP066837
• Organism: Escherichia coli strain Ec-FL1-1X

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JH476_RS25125
• Protein ID: WP_001372321.1
• Coordinates: 101909..102034 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	102111..102335 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH476_RS25090 (97278)	97278..97967	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
JH476_RS25095 (98154)	98154..98537	-	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JH476_RS25100 (98858)	98858..99460	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
JH476_RS25105 (99757)	99757..100578	-	822	WP_001234469.1	DUF932 domain-containing protein	-
JH476_RS25110 (100700)	100700..100987	-	288	WP_000107535.1	hypothetical protein	-
JH476_RS25115 (101012)	101012..101218	-	207	WP_000547939.1	hypothetical protein	-
JH476_RS26050 (101288)	101288..101461	+	174	Protein_102	hypothetical protein	-
JH476_RS26055 (101459)	101459..101689	-	231	WP_071587244.1	hypothetical protein	-
JH476_RS25125 (101909)	101909..102034	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JH476_RS26060 (101976)	101976..102125	-	150	Protein_105	plasmid maintenance protein Mok	-
- (102111)	102111..102335	-	225	NuclAT_0	-	Antitoxin
- (102111)	102111..102335	-	225	NuclAT_0	-	Antitoxin
- (102111)	102111..102335	-	225	NuclAT_0	-	Antitoxin
- (102111)	102111..102335	-	225	NuclAT_0	-	Antitoxin
JH476_RS25130 (102147)	102147..102335	+	189	WP_001299721.1	hypothetical protein	-
JH476_RS25135 (102304)	102304..103066	-	763	Protein_107	plasmid SOS inhibition protein A	-
JH476_RS25140 (103063)	103063..103497	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
JH476_RS25145 (103552)	103552..105510	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
JH476_RS25150 (105576)	105576..105809	-	234	WP_000005990.1	DUF905 family protein	-
JH476_RS25155 (105872)	105872..106369	-	498	WP_089552049.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185164 WP_001372321.1 NZ_CP066837:c102034-101909 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185164 NZ_CP088022:5444618-5444914 [Bradyrhizobium quebecense]
ATGATCCTGAGCTTCCGGGACGAATGGCTGTGCGCTTTCTTCACGGAGGACGCGAAATCGCGAAACATCCCGTCGGATCT
TGAAGACCGTTTGTTTCGCAAGCTGCAGATGCTCGATGATGCGACGACTGATCAGGACCTGCGGGTGCCGCCGAGCAATC
ATTTTGAGAAACTCCGGGGCAATCTTGCGGGGCTTCATTCCATTCGCGTCAACAAGCAATGGCGCCTTGTCTTTCGGTGG
TCGGGCGCGCGAGGCGAAGCACAAGGCGTCTACCTCGACGACCACAGTTACAGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT185164 NZ_CP066837:c102335-102111 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin