Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3982695..3983422 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JH476_RS19145 Protein ID WP_000550189.1
Coordinates 3982695..3983009 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag JH476_RS19150 Protein ID WP_000560253.1
Coordinates 3983006..3983422 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS19125 (3978861) 3978861..3979847 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
JH476_RS19130 (3979926) 3979926..3980609 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
JH476_RS19135 (3980686) 3980686..3981189 - 504 WP_001309773.1 M48 family metallopeptidase -
JH476_RS19140 (3981274) 3981274..3982410 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JH476_RS19145 (3982695) 3982695..3983009 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JH476_RS19150 (3983006) 3983006..3983422 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JH476_RS19155 (3983467) 3983467..3985485 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JH476_RS19160 (3985656) 3985656..3988007 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T185160 WP_000550189.1 NZ_CP066836:3982695-3983009 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T185160 NZ_CP088019:c3988944-3988528 [Pectobacterium carotovorum]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGTGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAGTGCACGTTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGCA
AAAAAGCCTCGCCACGCCATATGCAACTAGTTGATGCTTTCTGTCGCCGGCTTGATGCGGTGTTACCTTGGGATAGCGCC
GCAGTAGACGCTACGACGAAAATTAAAATCGCGCTAGCCGCAGCAGGCACGCCTATAGGCCCGAATGATACGGCCATCGC
GGGTCATGCCATCAGTGCTAAGGCCATATTGGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT185160 WP_000560253.1 NZ_CP066836:3983006-3983422 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT185160 NZ_CP088019:c3989168-3988941 [Pectobacterium carotovorum]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCACGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTTCGTCCGGTCAAACCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References