185159

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3939647..3940446 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag JH476_RS18920 Protein ID WP_000347269.1
Coordinates 3939647..3940111 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag JH476_RS18925 Protein ID WP_001309780.1
Coordinates 3940111..3940446 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS18890 (3934648) 3934648..3935082 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JH476_RS18895 (3935100) 3935100..3935978 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JH476_RS18900 (3935968) 3935968..3936747 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JH476_RS18905 (3936758) 3936758..3937231 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JH476_RS18910 (3937254) 3937254..3938534 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
JH476_RS18915 (3938783) 3938783..3939592 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JH476_RS18920 (3939647) 3939647..3940111 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JH476_RS18925 (3940111) 3940111..3940446 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JH476_RS18930 (3940595) 3940595..3942166 - 1572 WP_001273738.1 galactarate dehydratase -
JH476_RS18935 (3942541) 3942541..3943875 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JH476_RS18940 (3943891) 3943891..3944661 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T185159 WP_000347269.1 NZ_CP066836:c3940111-3939647 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T185159 NZ_CP088019:c3150772-3150458 [Pectobacterium carotovorum]
ATGCAATTTACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTTGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCAGACTGACGGATGGCAAAGAATACGCCGTAATGACTCACGAGCTGGCAAGTATCCCTGTCCAGGCACTGGGT
GCGGTGTTTTGTGATGCTTCTCAGTACCGTACTCAGGTAAAGGCTGCCATCGATTTCCTCATTGACGGGTTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT185159 WP_001309780.1 NZ_CP066836:c3940446-3940111 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT185159 NZ_CP088019:c3151011-3150775 [Pectobacterium carotovorum]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCTCGTGAGGCGGG
TATTAATCTGAGTGCTACCCTGACAACCGCCCTGGATGCAGAGCTTCGCTATCATGAAGCGAAAAAATGGCAAGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAACAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References