185147

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 718432..718994 Replicon chromosome
Accession NZ_CP066836
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JH476_RS03570 Protein ID WP_000605676.1
Coordinates 718432..718710 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JH476_RS03575 Protein ID WP_000781370.1
Coordinates 718710..718994 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS03545 (714018) 714018..714449 - 432 WP_000152305.1 peroxiredoxin OsmC -
JH476_RS03550 (714794) 714794..715009 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JH476_RS03555 (715111) 715111..715248 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JH476_RS03560 (715405) 715405..717102 + 1698 WP_024241319.1 malate dehydrogenase -
JH476_RS03565 (717236) 717236..718246 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JH476_RS03570 (718432) 718432..718710 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JH476_RS03575 (718710) 718710..718994 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JH476_RS03580 (719222) 719222..719875 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
JH476_RS03585 (719868) 719868..720752 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
JH476_RS03590 (720765) 720765..723812 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T185147 WP_000605676.1 NZ_CP066836:718432-718710 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T185147 NZ_CP088019:151584-152066 [Pectobacterium carotovorum]
ATGGGAATAACTGCACCAGAGCCATTATCCTCCGGCCATAATCTTGCTGAATTTTGCAGCCAAGAACCGAGTTTAAATGA
ATGGCTAAAAAAGAAAGCGCTAAAAAATCATAGTGCAGGGATTTCCCGTGTATATGTTATTTGCGCTGAAGGCACCCATC
AGGTGATTGGATATTATTGTTTATCAACAGGTAGCGTTCAGAGAAATACAGCACCTGGTGCATATAGGCGTAATGCACCT
GAATCACTACCAGTCATTGTCCTTGGGCGGCTTGCCATTGATCAGGCGTATTCTGGTAAAGGTTTGGGTGTGGCTTTATT
AAAAGACGCGATATTTCGGACTGAGAATATTGCTTTGCAAGTGGGTGTGAGGGCATTAGTCGTTCACGCTCTGGATGAGA
ACGTGCGCAATTTCTACCTTAAATTCGCTTTTGATCCCTCGCCAGTGCAGCCCCTGATGCTACTCTACCCAATTAAAATT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT185147 WP_000781370.1 NZ_CP066836:718710-718994 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT185147 NZ_CP088019:151315-151593 [Pectobacterium carotovorum]
ATGAAACCTGAAACTAAAGAAGCGCCAATCAATATTCGGGCCAAAGCCTCCCAGCGGGACTTGATCGATATGGCTGCAAA
CCTTGTGTCAAAATCACGCACCGATTTTATGCTTGAAGCCGCCTGCCGCGAGGCGCAGGACATTTTGTTGGATCAACGTT
TGTTTATTCTGGACAACGAACAGTTTGACGAATTTTTGGCCGAGTTGGATGCACCTATCACCACAGAGCGTCAGGCTCGC
ATTAACGATCTGATGAGCCGCAAATCTCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References