Detailed information of TA system TA185133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3277364..3277962
• Replicon: chromosome
• Accession: NZ_CP066833
• Organism: Proteus mirabilis strain RGF134-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: JEQ15_RS15265
• Protein ID: WP_001896384.1
• Coordinates: 3277364..3277687 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JEQ15_RS15270
• Protein ID: WP_000058216.1
• Coordinates: 3277684..3277962 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ15_RS15235 (JEQ15_15180)	3273077..3273463	-	387	WP_032469918.1	TraA family conjugative transfer protein	-
JEQ15_RS15240 (JEQ15_15185)	3273460..3274032	-	573	WP_148039504.1	type IV conjugative transfer system lipoprotein TraV	-
JEQ15_RS15245 (JEQ15_15190)	3274107..3275396	-	1290	WP_159262611.1	TraB/VirB10 family protein	-
JEQ15_RS15250 (JEQ15_15195)	3275399..3276295	-	897	WP_159262630.1	type-F conjugative transfer system secretin TraK	-
JEQ15_RS15255 (JEQ15_15200)	3276279..3276905	-	627	WP_159262613.1	TraE/TraK family type IV conjugative transfer system protein	-
JEQ15_RS15260 (JEQ15_15205)	3276902..3277183	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
JEQ15_RS15265 (JEQ15_15210)	3277364..3277687	+	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JEQ15_RS15270 (JEQ15_15215)	3277684..3277962	+	279	WP_000058216.1	helix-turn-helix transcriptional regulator	Antitoxin
JEQ15_RS15275 (JEQ15_15220)	3277988..3278623	-	636	WP_000033756.1	DUF4400 domain-containing protein	-
JEQ15_RS15280 (JEQ15_15225)	3278610..3279170	-	561	WP_159262615.1	conjugative transfer protein	-
JEQ15_RS15285 (JEQ15_15230)	3279180..3281000	-	1821	WP_000661889.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J)	-	3179172..3351656	172484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T185133 WP_001896384.1 NZ_CP066833:3277364-3277687 [Proteus mirabilis]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T185133 NZ_CP088007:6795506-6795925 [Nocardia huaxiensis]
ATGGGAGTAACCGAGTGGCTCATCGACAAGTCAGCGCTGGTGCGGCTTTCCGGATCGCCGGATATCGATGTCTGGGCGGA
ACGCATCGACCAGGGTCGCGTTCGAGTAGCGACAGTCACCAGGCTCGAGGTGGGATTTTCGGCTCGATCTGGACCAGATC
ACCGCGCGGCGTTCTCCCGGCCGCCTCTGTCGTCATTCCTCGTCGAGTACCTGACTCCGACCATGGAGGACCGAGCGCTC
GAGGTTCAGGCCGCACTCGCCGACGCCGGGCATCATCGCGCGCCGTCTGTCGCAGATCTGCTGGTTGCCGCTGTGGCCGA
GTTGTCAGGTCTGACGGTGCTGCATCTGGACAAGGACTTCGAGCTGATCGCCGACGTCACGGGTCAACCTGTGGAGCGAT
TACGGCTCTCGGGTGAATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT185133 WP_000058216.1 NZ_CP066833:3277684-3277962 [Proteus mirabilis]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT185133 NZ_CP088007:6795291-6795515 [Nocardia huaxiensis]
ATGTCGGCAGATCTGTTGATCCGGGACGTACCGGATGATGTCATCGCCGCGATCGATGCCAAGGCCAAACGCCTAGGTCT
ATCGCGGACGCAATATCTTCGCCGCACGCTGGCTCGCGAAGCGGCATCCGATGAACGGCGTGTCACCGCTGCGGATCTCG
TATGGTTCTCGGACACCTTTGCGGATCTCGCAGATGAGGAGGTAATGCGCCGCGCATGGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A244CN87