Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4740020..4740729 Replicon chromosome
Accession NZ_CP066812
Organism Mycobacterium avium subsp. paratuberculosis strain 42-13-1

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag MAPJR_RS22280 Protein ID WP_003873387.1
Coordinates 4740325..4740729 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag MAPJR_RS22275 Protein ID WP_003873388.1
Coordinates 4740020..4740328 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAPJR_RS22260 4735905..4736207 + 303 WP_003873391.1 co-chaperone GroES -
MAPJR_RS22265 4736296..4737912 + 1617 WP_202151982.1 chaperonin GroEL -
MAPJR_RS22270 4738007..4739533 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
MAPJR_RS22275 4740020..4740328 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
MAPJR_RS22280 4740325..4740729 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MAPJR_RS22285 4740969..4741661 + 693 WP_010950286.1 DUF4352 domain-containing protein -
MAPJR_RS22290 4741716..4742915 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
MAPJR_RS22295 4743072..4743836 - 765 WP_003874401.1 superoxide dismutase family protein -
MAPJR_RS22300 4743879..4744187 - 309 WP_003874400.1 WhiB family transcriptional regulator -
MAPJR_RS22305 4744585..4745415 + 831 WP_003874399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T185124 WP_003873387.1 NZ_CP066812:c4740729-4740325 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T185124 NZ_CP088000:c3709964-3709686 [Stenotrophomonas rhizophila]
ATGATCCAGTCCTTCCGCTGCCGCCATACGCTTGCCCTGTTCGAAGGCCGATGCCCACGGCGGTTCCGCAGCATCCAGGC
GGTTGCCGAGCGCAAACTGCAGATGCTGGACGCGGCGCAGACCCTGCAGTTCCTGCGCAGTCCGCCCGGCAACCGTCTTG
AAGCGCTGGCGGGCGAACGACGAGGGCAGTTCAGCATCCGCATCAATGTCCAATGGCGCGTGTGTTTCACCTGGACGGAT
GCAGGTCCGTGCCATGTCGAAATCGTTGATTACCACTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT185124 WP_003873388.1 NZ_CP066812:c4740328-4740020 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT185124 NZ_CP088000:c3709674-3709372 [Stenotrophomonas rhizophila]
ATGAGCCGTGCCGCCAACAAACTGAGGGCCATCCACCCGGGCGAAATCCTGCGGGAGGAATTCATGGCGCCGCTCGAGCT
GAGCATCAATGCGCTCGCGCGTGCCCTGGACGTGCCGGCCACCCGGCTGCATGCCATCGTGCATGAAACCCGGGGCGTCA
CCGCGGATACGGCGGCCCGGCTGGCGCGCCATTTCGGCGGTGACGCCGCCTCGTGGCTCGCACTGCAGGCCGAATACGAC
CTCAAGACCCTGTCTACGTGGCAGGACATCGAACGCAGGGTCATGCCGCGCGTTGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References