Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3517075..3517790 Replicon chromosome
Accession NZ_CP066809
Organism Shigella flexneri 3a strain 888048

Toxin (Protein)


Gene name yafO Uniprot ID A0A658YZ27
Locus tag JGY96_RS17955 Protein ID WP_001263492.1
Coordinates 3517075..3517494 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag JGY96_RS17960 Protein ID WP_000554759.1
Coordinates 3517497..3517790 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGY96_RS17930 (3512379) 3512379..3513836 + 1458 WP_025759252.1 cytosol nonspecific dipeptidase -
JGY96_RS17935 (3513893) 3513893..3514453 - 561 Protein_3504 peptide chain release factor H -
JGY96_RS17940 (3514569) 3514569..3515613 - 1045 Protein_3505 RNA ligase RtcB family protein -
JGY96_RS17945 (3515859) 3515859..3516311 - 453 WP_001059862.1 GNAT family N-acetyltransferase -
JGY96_RS17950 (3516788) 3516788..3516789 - 2 WP_094110542.1 IS1 family transposase -
JGY96_RS17955 (3517075) 3517075..3517494 - 420 WP_001263492.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JGY96_RS17960 (3517497) 3517497..3517790 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JGY96_RS17965 (3517842) 3517842..3518897 - 1056 WP_001226172.1 DNA polymerase IV -
JGY96_RS17970 (3518968) 3518968..3519753 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
JGY96_RS17975 (3519725) 3519725..3521437 + 1713 Protein_3512 flagellar biosynthesis protein FlhA -
JGY96_RS17980 (3521654) 3521654..3522151 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16348.95 Da        Isoelectric Point: 8.0871

>T185119 WP_001263492.1 NZ_CP066809:c3517494-3517075 [Shigella flexneri 3a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFWVMLPTY

Download         Length: 420 bp

>T185119 NZ_CP087999:4218782-4219036 [Geomonas sp. RF6]
ATGAGGGCAAGTATCATCCGTATCGGGAATTCTCAGGGGATAAGGATCCCAAAGCTCCTTCTTGAGCAAACGAGACTCGG
AAGTGAGGTTGAACTCGAAGTCGAAGACGACAAGATAGTGATTCGGCCGGTATCCCGACCACGACAGGGCTGGCTTGAAA
AGTTTAAACTCATGGCAGAGCAGGGTGACGACCGGCTGCTGGACCCGGGCGCTGGGGAAGAGACGGAGTGGGATAAGGAT
GAATGGGAATGGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT185119 WP_000554759.1 NZ_CP066809:c3517790-3517497 [Shigella flexneri 3a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT185119 NZ_CP087999:4219030-4219353 [Geomonas sp. RF6]
ATGGTGACAAAGCGCTTCGAGGTCTATCTGGTGAATCTTGATCCGACTGTCGGAAGTGAGATTCGGAAGGCCCGACCGTG
TCTCATAATCTCTCCGGACGAGATGAACCGTTTTCTTGCAACTGTAATTGTTGCGCCGATGACCACTAAGGGAAGGGATT
ACCCAACGCGGGTACCTTGCTCGTTCAAAGGAAAGGACGGGCACGTAGTGCTTGATCAACTGAGAACGGTGGACAAGATC
CGGCTCGTGCAAAAGCTTGGAAAGGTGAAACCACAAACTCAGCAGGATGTGCTGGCAGTGTTGTCCGACATGTTCGCGGA
GTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A658YZ27

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References