Detailed information of TA system TA185116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3111022..3111550
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JGY96_RS15890
• Protein ID: WP_236743520.1
• Coordinates: 3111022..3111252 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: JGY96_RS15895
• Protein ID: WP_000147081.1
• Coordinates: 3111242..3111550 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS15860 (3106539)	3106539..3106753	+	215	Protein_3103	transposase domain-containing protein	-
JGY96_RS15865 (3106784)	3106784..3108031	-	1248	Protein_3104	replicative DNA helicase	-
JGY96_RS15870 (3108028)	3108028..3108906	-	879	WP_000988187.1	ATP-binding protein	-
JGY96_RS15875 (3108917)	3108917..3109824	-	908	Protein_3106	DNA-binding protein	-
JGY96_RS15880 (3109811)	3109811..3110044	-	234	WP_000618004.1	hypothetical protein	-
JGY96_RS15885 (3110041)	3110041..3111009	-	969	WP_000961310.1	Rha family phage regulatory protein	-
JGY96_RS15890 (3111022)	3111022..3111252	+	231	WP_236743520.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGY96_RS15895 (3111242)	3111242..3111550	+	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
JGY96_RS15900 (3111592)	3111592..3111768	-	177	WP_001254220.1	NinE family protein	-
JGY96_RS15905 (3111765)	3111765..3112175	-	411	WP_000814621.1	recombination protein NinB	-
JGY96_RS15910 (3112172)	3112172..3112450	-	279	WP_025757756.1	hypothetical protein	-
JGY96_RS15915 (3112525)	3112525..3114873	-	2349	Protein_3114	toprim domain-containing protein	-
JGY96_RS15920 (3114913)	3114913..3116074	-	1162	Protein_3115	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH4.5 / ipaH9.8	3059007..3132089	73082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8695.15 Da
• Isoelectric Point: 10.5308

>T185116 WP_236743520.1 NZ_CP066809:3111022-3111252 [Shigella flexneri 3a]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T185116 NZ_CP087999:1358886-1359128 [Geomonas sp. RF6]
TTGTCCAAGAGCGCCGGGAACAAGCAAAAGCACGGCATCGATTTCGACGAAGCCCAAAGTCTCTGGGATGACCCGGATCT
GCTTGAGGTCCCTGTTGTAACCACCGATGGACCTCGTTTTCTTGTGGTCGGCAGGATTTCGGGAAAGCACTGGTCTGGCA
TCATTACGTACCGCGGCGATGCCATCAGAATCATCTCGGTCCGGCGCTCCCGCAAGGAGGAAATTGCACTATATGAAAGC
TGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT185116 WP_000147081.1 NZ_CP066809:3111242-3111550 [Shigella flexneri 3a]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT185116 NZ_CP087999:1359118-1359342 [Geomonas sp. RF6]
ATGAAAGCTGATGACATAGACAAGACTTTCGACGAAGGTGGCGATATCTCTGCACATCTCGATGTGACGAAGGCGAGAAG
GCCGGAACAGGAACAAAAGCGGGTCAATGTTGACTTTCCCCTCTGGATGATCCAGCAACTTGACAAGGAATCACGGCGCC
TGGGTGTCCCGCGGCAGGCCATCATCAAGGTATGGGTGGCGGAACGCCTGGAGAAAGCCGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1