Detailed information of TA system TA185112

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2274458..2274683
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: JGY96_RS11480
• Protein ID: WP_000813254.1
• Coordinates: 2274458..2274613 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2274625..2274683 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS11450	2269721..2270134	+	414	WP_254100537.1	hypothetical protein	-
JGY96_RS11455	2270085..2271271	+	1187	Protein_2237	IS91 family transposase	-
JGY96_RS11460	2271682..2272236	-	555	WP_000640143.1	DUF1133 family protein	-
JGY96_RS11465	2272233..2272523	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
JGY96_RS11470	2272523..2273122	-	600	WP_000940329.1	DUF1367 family protein	-
JGY96_RS11475	2273256..2273952	+	697	WP_254100540.1	IS1 family transposase	-
JGY96_RS11480	2274458..2274613	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2274625..2274683	+	59	-	-	Antitoxin
JGY96_RS11485	2275068..2275433	-	366	WP_000610655.1	DUF551 domain-containing protein	-
JGY96_RS11490	2275433..2276098	-	666	WP_005115317.1	hypothetical protein	-
JGY96_RS11495	2276098..2276460	-	363	Protein_2245	HNH endonuclease	-
JGY96_RS11500	2276462..2276680	-	219	WP_000256997.1	DUF4014 family protein	-
JGY96_RS11505	2276773..2277129	-	357	WP_000403784.1	hypothetical protein	-
JGY96_RS11510	2277187..2277609	-	423	WP_001118171.1	DUF977 family protein	-
JGY96_RS11515	2277624..2278370	-	747	WP_000788996.1	ATP-binding protein	-
JGY96_RS11520	2278516..2278685	-	170	Protein_2250	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2252957..2292731	39774

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T185112 WP_000813254.1 NZ_CP066809:c2274613-2274458 [Shigella flexneri 3a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T185112 NZ_CP087997:c3718705-3718454 [Subtercola sp. AK-R2A1-2]
ATGACCGCATTGACCGCGACGGAAGCACGTAAGACCTTGTTCGGGCTCATTCAGCAAGTCAACGACGATCACACGGCCGT
CGAAGTTGTATCCAAGCATGGCAACGCTGTGCTCATGTCGAAAGACGACTACGACGCCATGACCGAGACCGCTTACCTGC
TGCGCAACCCAGCAAATGCGGAACGCTTGCTCGAATCAATCGAGCGCGCCAGGCGAGGCGAGTTCGAAAAGCACGACCTC
CTTGACGCGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT185112 NZ_CP066809:2274625-2274683 [Shigella flexneri 3a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin