Detailed information of TA system TA185102

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1784578..1784803
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: JGY96_RS08765
• Protein ID: WP_000813254.1
• Coordinates: 1784648..1784803 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1784578..1784636 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS08730	1780163..1780960	-	798	WP_001325918.1	DgsA anti-repressor MtfA	-
JGY96_RS08740	1781171..1782208	-	1038	WP_004974968.1	tyrosine-type recombinase/integrase	-
JGY96_RS08745	1782208..1782411	-	204	WP_025759339.1	DUF4224 domain-containing protein	-
JGY96_RS08750	1782470..1782774	-	305	Protein_1709	3'-5' exoribonuclease	-
JGY96_RS08755	1783256..1783257	-	2	WP_153933088.1	IS1 family transposase	-
JGY96_RS08760	1783580..1783996	+	417	WP_005069274.1	hypothetical protein	-
-	1784578..1784636	-	59	-	-	Antitoxin
JGY96_RS08765	1784648..1784803	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
JGY96_RS08770	1784971..1785249	+	279	WP_000929754.1	hypothetical protein	-
JGY96_RS08775	1785251..1786309	+	1059	WP_025759819.1	DUF968 domain-containing protein	-
JGY96_RS08780	1786310..1786675	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
JGY96_RS08785	1786672..1787387	+	716	Protein_1716	bacteriophage antitermination protein Q	-
JGY96_RS08810	1788183..1788398	+	216	WP_000839572.1	class II holin family protein	-
JGY96_RS08815	1788497..1789171	+	675	WP_001088287.1	IS66-like element accessory protein TnpA	-
JGY96_RS08820	1789168..1789515	+	348	WP_000631711.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gnd / ugd / ipaH9.8	1711270..1828915	117645
	-	inside	IScluster/Tn	-	-	1767062..1792398	25336

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T185102 WP_000813254.1 NZ_CP066809:1784648-1784803 [Shigella flexneri 3a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T185102 NZ_CP087994:c3337677-3337417 [Acetobacterium wieringae]
ATGAGTAATCTGACCTTTTCGGATAAGGGCTGGGAAGACTATCTCTATTGGCAATTGACCGATAAAAAGATGTTGCGGAG
AATAAATGAATTGCTAAAAGATATTCAACGAAATGGCTATACCGGAATTGGTAAGCCTGAGCCCCTGAAGTATCGTAAAG
CATGGAGCCGGCGCATCGATTTGGAACACCGTCTGGTCTATGCCCTGGATGAAGATGGAAATATTTATATTCTTTCGCTC
AAAGGACATTATCAGGATTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT185102 NZ_CP066809:c1784636-1784578 [Shigella flexneri 3a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin