Detailed information of TA system TA185101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1013536..1014263
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2Y4XNA6
• Locus tag: JGY96_RS05000
• Protein ID: WP_000547562.1
• Coordinates: 1013536..1013847 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JGY96_RS05005
• Protein ID: WP_000126288.1
• Coordinates: 1013844..1014263 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS04970 (1008694)	1008694..1010379	+	1686	WP_025758592.1	formate hydrogenlyase subunit HycE	-
JGY96_RS04975 (1010389)	1010389..1010931	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
JGY96_RS04980 (1010931)	1010931..1011698	+	768	WP_025758590.1	formate hydrogenlyase subunit HycG	-
JGY96_RS04985 (1011695)	1011695..1012105	+	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
JGY96_RS04990 (1012098)	1012098..1012568	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
JGY96_RS04995 (1012620)	1012620..1013363	+	744	WP_024259998.1	hypothetical protein	-
JGY96_RS05000 (1013536)	1013536..1013847	+	312	WP_000547562.1	type II toxin-antitoxin system HigB family toxin	Toxin
JGY96_RS05005 (1013844)	1013844..1014263	+	420	WP_000126288.1	helix-turn-helix domain-containing protein	Antitoxin
JGY96_RS05010 (1014378)	1014378..1015802	-	1425	WP_119182051.1	6-phospho-beta-glucosidase AscB	-
JGY96_RS05015 (1015811)	1015811..1017268	-	1458	WP_001107865.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
JGY96_RS05020 (1017528)	1017528..1018538	+	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
JGY96_RS05025 (1018687)	1018687..1019214	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4924

>T185101 WP_000547562.1 NZ_CP066809:1013536-1013847 [Shigella flexneri 3a]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T185101 NZ_CP087994:2087138-2087413 [Acetobacterium wieringae]
ATGAAATACGAAGTTAAATTCACAAATCAGTTTAAAAAAGATCTTAAGCTTGCCAAAAAACAAGGCAGAGATATTGACCG
ACTTTATGCTGTTATTGAAAAACTTGCCGATGATGAACCTCTGGAACAAAAATATCGTGATCATGATTTAAGTGGTAATT
ATAAAGGATGCCGTGAATGTCATGTCGAACCCGATTGGCTTTTGGTCTATGAGGTGATGGACGATGTTCTGGTATTAATG
CTTTATCGTATCGGAAGTCACTCAACGTTGTTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15397.33 Da
• Isoelectric Point: 4.3648

>AT185101 WP_000126288.1 NZ_CP066809:1013844-1014263 [Shigella flexneri 3a]
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT185101 NZ_CP087994:2086866-2087141 [Acetobacterium wieringae]
ATGGCTACTAGTACAAATTTGAATATTCGTACCGATAAAGATATCAAAGAAGCTGCAGAGAAAATTTTTGGAGAGCTTGG
TCTTAACATGACAACAGCAATCAATATTTTTCTCAGACAAGCAATCCGGGAAAATGGGATACCTTTTGAAATGAAATTGT
CTGTTCCAAATCAGGAAACAGTTGCTGCCATTACCGAAGGTCGAAAACTGGCCAAAGATAGCAGTGTCAAAGGTTATTCC
AATATGGATGATTTGCGAGCTGCACTGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A2Y4XNA6