Detailed information of TA system TA185099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 643422..644149
• Replicon: chromosome
• Accession: NZ_CP066809
• Organism: Shigella flexneri 3a strain 888048

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JGY96_RS03185
• Protein ID: WP_119182032.1
• Coordinates: 643422..643736 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JGY96_RS03190
• Protein ID: WP_000560266.1
• Coordinates: 643733..644149 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY96_RS03165 (639567)	639567..640565	-	999	WP_005050984.1	Gfo/Idh/MocA family oxidoreductase	-
JGY96_RS03170 (640644)	640644..641336	-	693	WP_000942537.1	vancomycin high temperature exclusion protein	-
JGY96_RS03175 (641413)	641413..641916	-	504	WP_005071012.1	M48 family metallopeptidase	-
JGY96_RS03180 (642001)	642001..643137	+	1137	WP_000018657.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
JGY96_RS03185 (643422)	643422..643736	+	315	WP_119182032.1	type II toxin-antitoxin system toxin HigB	Toxin
JGY96_RS03190 (643733)	643733..644149	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
JGY96_RS03195 (644194)	644194..646212	-	2019	WP_040235166.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
JGY96_RS03200 (646438)	646438..648789	-	2352	WP_000695506.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12077.07 Da
• Isoelectric Point: 10.1027

>T185099 WP_119182032.1 NZ_CP066809:643422-643736 [Shigella flexneri 3a]
MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T185099 NZ_CP087994:c116478-116227 [Acetobacterium wieringae]
ATCAATGATTACATCCAAATGCTCAGTAAATACGGTACAAAGATCGGCGAGCCATACATCAAGCATTTAGAGGGCGAAAT
TTGGGAGATCAGGCCCTTAAGAAATAGAATCTTCTTCGTCGCCTGGGTAGATGGTAGTTATGTATTGCTACACCAATTTA
TAAAGAAATCACAAAAGACCCCTAAACGAGAAATCGAACAAGCCAAACGAGAATTGGCATTCTTAAAAGAAAGTGGTGAA
AATAATGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT185099 WP_000560266.1 NZ_CP066809:643733-644149 [Shigella flexneri 3a]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT185099 NZ_CP087994:c116234-115875 [Acetobacterium wieringae]
ATGAATAATAAAGCAATTGGTAGTAGCTGGACCGATGTCAGAAAAGAAATTTTTACACCTGAAGAAATTCTGGAATCAGA
TTTAAGGGTTGCTCTCATGGGTGAAATCATTAAAGCACGCCATGATAAAGGGATTAGCCAGAAAAAGCTTGAAGAATTAA
GTGGTGTCAGACAGCCAGTGATTGCCAGAATGGAAAGAGGTTCTACCGATCCCAAATTAACAACAGTCCTGAAAATTTTG
GAACCGTTAGGAATGACTTTGACGATTGTGCCCATAAAGGAAAAACGCATGCGTCTTAAACCGCCGTTTGAACTCAATCT
TTCTAAAAATGGCGGTAAACATCCTATAAAAAGAGGCTAG