Detailed information of TA system TA185094

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70526..70779
• Replicon: plasmid unnamed1
• Accession: NZ_CP066807
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JGU77_RS24210
• Protein ID: WP_001312851.1
• Coordinates: 70630..70779 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	70526..70585 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS24175	66302..67162	+	861	WP_000704523.1	alpha/beta hydrolase	-
JGU77_RS24180	67265..67825	+	561	WP_000139321.1	fertility inhibition protein FinO	-
JGU77_RS24185	67954..68166	+	213	WP_001309245.1	ANR family transcriptional regulator	-
JGU77_RS24190	68411..68872	+	462	WP_001233838.1	thermonuclease family protein	-
JGU77_RS24195	68918..69127	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
JGU77_RS24200	69165..69755	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
JGU77_RS24205	69910..70383	+	474	WP_016240489.1	hypothetical protein	-
-	70526..70585	-	60	NuclAT_1	-	Antitoxin
-	70526..70585	-	60	NuclAT_1	-	Antitoxin
-	70526..70585	-	60	NuclAT_1	-	Antitoxin
-	70526..70585	-	60	NuclAT_1	-	Antitoxin
JGU77_RS24210	70630..70779	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
JGU77_RS24215	71064..71312	+	249	WP_000083839.1	replication regulatory protein RepA	-
JGU77_RS24220	71557..71631	+	75	WP_001365705.1	RepA leader peptide Tap	-
JGU77_RS24225	71624..72481	+	858	WP_000130961.1	incFII family plasmid replication initiator RepA	-
JGU77_RS24230	73182..73394	+	213	WP_032155400.1	hypothetical protein	-
JGU77_RS24235	73509..73817	-	309	Protein_91	transposase	-
JGU77_RS24240	74010..74108	+	99	WP_137491101.1	transposase	-
JGU77_RS24245	74258..74506	-	249	WP_000738203.1	hypothetical protein	-
JGU77_RS24250	74536..75306	-	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C	-	1..131861	131861
	-	flank	IS/Tn	-	-	73509..73775	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185094 WP_001312851.1 NZ_CP066807:70630-70779 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185094 NZ_CP087993:1331772-1332065 [Xylella fastidiosa subsp. multiplex]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185094 NZ_CP066807:c70585-70526 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin