Detailed information of TA system TA185090

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28999..29425
• Replicon: plasmid unnamed1
• Accession: NZ_CP066807
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JGU77_RS23960
• Protein ID: WP_001312861.1
• Coordinates: 29267..29425 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	28999..29223 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS23925	24058..24279	+	222	WP_042111886.1	hypothetical protein	-
JGU77_RS23930	24690..24896	+	207	WP_000275853.1	hypothetical protein	-
JGU77_RS23935	24922..25461	+	540	WP_097498141.1	single-stranded DNA-binding protein	-
JGU77_RS23940	25524..25758	+	235	Protein_32	DUF905 family protein	-
JGU77_RS23945	25824..27782	+	1959	WP_000117163.1	ParB/RepB/Spo0J family partition protein	-
JGU77_RS23950	27837..28271	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JGU77_RS23955	28268..28987	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	28999..29223	+	225	NuclAT_0	-	Antitoxin
-	28999..29223	+	225	NuclAT_0	-	Antitoxin
-	28999..29223	+	225	NuclAT_0	-	Antitoxin
-	28999..29223	+	225	NuclAT_0	-	Antitoxin
JGU77_RS23960	29267..29425	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JGU77_RS23965	29726..29930	-	205	Protein_37	pilus protein	-
JGU77_RS23970	30322..30618	+	297	WP_001272251.1	hypothetical protein	-
JGU77_RS23975	30729..31550	+	822	WP_001234445.1	DUF945 domain-containing protein	-
JGU77_RS23980	31847..32437	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
JGU77_RS23985	32770..33153	+	384	WP_001151529.1	relaxosome protein TraM	-
JGU77_RS23990	33345..33992	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
JGU77_RS23995	34100..34339	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C	-	1..131861	131861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T185090 WP_001312861.1 NZ_CP066807:29267-29425 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T185090 NZ_CP087992:c2471280-2470852 [Xylella fastidiosa subsp. multiplex]
ATGATTGTCTTGGATACCAATGTTGTTTCCGAAGCAATGAAACCCGAGCCGGATGCAGCAGTCCGAACCTGGCTCAACGA
GCAGATGAGCGTCACGTTGTACCTATCCAGTGTGACGTTATCTGAACTGTTGTTTGGAATCGCAGTGTTGCCCACAAGTA
AGCGCAAGGACATGTTGGCTCGTACCCTTGATGGCTTGCTTGATCTGTTCAATGAACGGGTGTTGCCGTTCGATACGGAC
GCGGCACGCCACTATGCTGAACTGGCAGTGAAAGCGAGAAACAATGGGCGCGGCTTTCCCACACCGGATGGCTATATCGC
GGCAATTGCTGCATCACGAGGTTACATAGTAGCTTCCCGCGACACCTCAGCCTATGAGTCAAGTGGTGTGCAAGTCATCA
ATCCGTGGCAATACAGCAAACAAACATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT185090 NZ_CP066807:28999-29223 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGGTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin