Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3894589..3895283 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JGU77_RS18595 Protein ID WP_001263491.1
Coordinates 3894589..3894987 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JGU77_RS18600 Protein ID WP_000554755.1
Coordinates 3894990..3895283 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS18565 3889851..3891308 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
JGU77_RS18570 3891356..3891598 + 243 WP_001029361.1 hypothetical protein -
JGU77_RS18575 3891615..3892124 - 510 WP_001361775.1 hydrolase -
JGU77_RS18580 3892186..3892800 - 615 WP_075862064.1 peptide chain release factor H -
JGU77_RS18585 3892797..3893936 - 1140 WP_170990260.1 RNA ligase RtcB family protein -
JGU77_RS18590 3894127..3894579 - 453 WP_075862062.1 GNAT family N-acetyltransferase -
JGU77_RS18595 3894589..3894987 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JGU77_RS18600 3894990..3895283 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JGU77_RS18605 3895335..3896390 - 1056 WP_075862061.1 DNA polymerase IV -
JGU77_RS18610 3896461..3897246 - 786 WP_179145761.1 putative lateral flagellar export/assembly protein LafU -
JGU77_RS18615 3897218..3898930 + 1713 Protein_3647 flagellar biosynthesis protein FlhA -
JGU77_RS18620 3899042..3899539 - 498 WP_000006244.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3894589..3910745 16156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T185082 WP_001263491.1 NZ_CP066806:c3894987-3894589 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T185082 NZ_CP087978:c5498883-5498500 [Klebsiella variicola subsp. variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT185082 WP_000554755.1 NZ_CP066806:c3895283-3894990 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT185082 NZ_CP087978:c5499125-5498883 [Klebsiella variicola subsp. variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References