Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2507128..2507690 Replicon chromosome
Accession NZ_CP066806
Organism Escherichia coli strain TCM3e1

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JGU77_RS11990 Protein ID WP_000605676.1
Coordinates 2507412..2507690 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JGU77_RS11985 Protein ID WP_000781370.1
Coordinates 2507128..2507412 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGU77_RS11970 2502487..2505534 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
JGU77_RS11975 2505547..2506431 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
JGU77_RS11980 2506424..2507077 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
JGU77_RS11985 2507128..2507412 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
JGU77_RS11990 2507412..2507690 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JGU77_RS11995 2507876..2508886 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JGU77_RS12000 2509020..2510717 - 1698 WP_000433464.1 malate dehydrogenase -
JGU77_RS12005 2510874..2511011 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JGU77_RS12010 2511113..2511328 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JGU77_RS12015 2511673..2512104 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T185069 WP_000605676.1 NZ_CP066806:c2507690-2507412 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T185069 NZ_CP087977:c128032-127754 [Janibacter limosus]
GTGATCGTCAGTTTCCGGCACAAGGGACTGGAGCGGTTGTACCGCACCGGGACGAAGAAGGGTGTCCAGGCGGCGCACGT
CCCCAAGCTGCTGCGCATCCTGTCGGCCCCGGACGTCGCCCAAGGACCGGCCGACCTTGAGATCCCGTCCTTTCGCACTC
ATCGACTCAAGGGTGACCTGACGGGCCACCACTCCATATGGGTCAACGGCAACTGGCGCATCACCTTCCGATTCATCGAG
TCGGATGTCGAGCTTGTCGATTATCAGGACTATCACTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT185069 WP_000781370.1 NZ_CP066806:c2507412-2507128 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT185069 NZ_CP087977:c127733-127449 [Janibacter limosus]
ATGATGAAGAATCCGGTCCACCCCGGCGAGATCCTGCGCGAGGACGTTTTGGCTGACCTCGGCCTCACCGTCAGCGAGGC
CGCCAATCGCCTCGGCGTCTCGCGGGTGTCGTTGAGCCGGGTCCTGCACGGCCACGCCGGGATCAGCTCCACCTTGGCGA
TCCGGCTCGAGGCTGCCGGCGTCGGCACCGCGAGCTCGTGGCTGGCCATGCAGTCTGCCCACGACCTGTCCGTCGAACGT
GCGGCGGGCATCCCCAAGGTGCAGCGCCTCACGTCGGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References