Detailed information of TA system TA185063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 658486..659285
• Replicon: chromosome
• Accession: NZ_CP066806
• Organism: Escherichia coli strain TCM3e1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JGU77_RS03195
• Protein ID: WP_000347273.1
• Coordinates: 658486..658950 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: JGU77_RS03200
• Protein ID: WP_001308975.1
• Coordinates: 658950..659285 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU77_RS03165	653487..653921	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JGU77_RS03170	653939..654817	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JGU77_RS03175	654807..655586	-	780	WP_075862220.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JGU77_RS03180	655597..656070	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JGU77_RS03185	656093..657373	-	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JGU77_RS03190	657622..658431	+	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
JGU77_RS03195	658486..658950	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JGU77_RS03200	658950..659285	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JGU77_RS03205	659434..661005	-	1572	WP_001273755.1	galactarate dehydratase	-
JGU77_RS03210	661380..662714	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JGU77_RS03215	662730..663500	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T185063 WP_000347273.1 NZ_CP066806:c658950-658486 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T185063 NZ_CP087952:c3597107-3596847 [Desulfosarcina sp. BuS5]
ATGAGCTTTGAATTTGACCAGAAAAAATCGGATACTAACAAAATCAAGCATGGGATTGATTTTGTTGAGGCTCAAGAGCT
TTTGAATGATGTTGATTTATTGGAGATCCCAGCAAAAACAATCGATGAATCGCGCTTCTTGGTCATTGGTAAAATAGGCG
AAAAGCACTGGGCTGGGATTACCACTTATCGAAATAATAATATTCGAATTATCTCAGCACGGCGTGCCAGAGATGAGGAG
ATTGAGCTTTATGAAAGCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT185063 WP_001308975.1 NZ_CP066806:c659285-658950 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT185063 NZ_CP087952:c3596857-3596627 [Desulfosarcina sp. BuS5]
ATGAAAGCTGAAGAATTAGATAAAAAGTTTGATGAAGGAAAAAGTATTTTAAAGAATTTTGACTTCTCAAAAGCAAGACG
GCTGAACCAGGAGCAAAAAAGGGTTAATGTCGATTTTCCTATATGGATGATTCAGTCTTTAGATAAAGAAGCTAAACGTC
TTGGCGTCCCTCGGCAATCCATTATTAAAATTTGGCTGGCGGAGAGATTGAACAAAACAGCCACCATATAG

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08