Detailed information of TA system TA184985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4384138..4384832
• Replicon: chromosome
• Accession: NZ_CP066759
• Organism: Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JG533_RS22080
• Protein ID: WP_001263495.1
• Coordinates: 4384138..4384536 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JG533_RS22085
• Protein ID: WP_000554757.1
• Coordinates: 4384539..4384832 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4379725..4379805	-	81	NuclAT_12	-	-
-	4379725..4379805	-	81	NuclAT_12	-	-
-	4379725..4379805	-	81	NuclAT_12	-	-
-	4379725..4379805	-	81	NuclAT_12	-	-
JG533_RS22055	4380401..4380859	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JG533_RS22060	4381120..4382577	+	1458	WP_001292995.1	cytosol nonspecific dipeptidase	-
JG533_RS22065	4382634..4383155	-	522	Protein_4322	peptide chain release factor H	-
JG533_RS22070	4383154..4383357	-	204	Protein_4323	RNA ligase RtcB family protein	-
JG533_RS22075	4383676..4384128	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JG533_RS22080	4384138..4384536	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JG533_RS22085	4384539..4384832	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JG533_RS22090	4384884..4385939	-	1056	WP_001226188.1	DNA polymerase IV	-
JG533_RS22095	4386010..4386795	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JG533_RS22100	4386767..4388479	+	1713	Protein_4329	flagellar biosynthesis protein FlhA	-
JG533_RS22105	4388624..4389121	-	498	Protein_4330	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T184985 WP_001263495.1 NZ_CP066759:c4384536-4384138 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T184985 NZ_CP087859:429879-430271 [Moraxella bovis]
ATGAAATATTTGCTGGACACCAATGCAGTCATTGCGATTTTGAATGCCAGTGAACGATTTTTGACCACGCTAGAACGGCA
TAAAGAGCGTGAAATTGCCATCTCGTCAATTGTTTTGTCGGAGCTGTATTATGGGGCGTACAAAAGCCAAAAAACCGCCC
AAAACCTTGAAAAAATCAATCTACTACCCTTTGAAGTGTTGCAATTTAATTCACAGGACGCTGATAAGGCTGGCGAAATT
GGTGCAACCCTAGAAAGGCGTGGCACACCCATTGGTTTTTATGACACGCTGATTGCAGGGCAAGCGATTGCCAATGATTT
GATTGTTATTACTGACAATGTGCGAGAATTTCTCCGAGTGGACGGTTTGCAAATTGAAAATTGGTTAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT184985 WP_000554757.1 NZ_CP066759:c4384832-4384539 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT184985 NZ_CP087859:429619-429882 [Moraxella bovis]
ATGCGCCAAACTGCCACCGCAAAATTATTTTGGACTGGCAACAGTCAAGCCGTTCGCTTACCCAAAGCCTTTCGTTTTGA
AGGCACGGAAGTGCGTATCAAAAAACAAGGACGACAAGTGATTATTGAGCCAGCAGAGACCCAAGATTGGGCGTGGCTGG
ACAAATTGCGAGCAATGCCAAAAGATGACAGTTTTGAAGAAGCCATTTTGGAAGGTCGCCAAAATTTAAAAGCCGATGAA
CGAGATTGGGGTGCATTTGAATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6