Detailed information of TA system TA184967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 652567..653366
• Replicon: chromosome
• Accession: NZ_CP066759
• Organism: Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JG533_RS03210
• Protein ID: WP_000347273.1
• Coordinates: 652567..653031 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JG533_RS03215
• Protein ID: WP_001302819.1
• Coordinates: 653031..653366 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG533_RS03180	647568..648002	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JG533_RS03185	648020..648898	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JG533_RS03190	648888..649667	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JG533_RS03195	649678..650151	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JG533_RS03200	650174..651454	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JG533_RS03205	651703..652512	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JG533_RS03210	652567..653031	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JG533_RS03215	653031..653366	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JG533_RS03220	653515..655086	-	1572	WP_001273776.1	galactarate dehydratase	-
JG533_RS03225	655461..656795	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JG533_RS03230	656811..657581	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T184967 WP_000347273.1 NZ_CP066759:c653031-652567 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T184967 NZ_CP087854:c2154581-2154180 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT184967 WP_001302819.1 NZ_CP066759:c653366-653031 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT184967 NZ_CP087854:c2154829-2154596 [Moraxella bovis]
ATGCAAACCAGTGTTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGCCAAATTTCGCTTTGATGACGATGTCAA
AGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATGGTGTGTGGGACAGCTTTTTTTTGAGTG
ATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGCGAGCGATTGACTTTTGACTGA

Structures

Toxin

• AlphaFold DB: V0SSH7