Detailed information of TA system TA184953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4374522..4375216
• Replicon: chromosome
• Accession: NZ_CP066756
• Organism: Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JG530_RS21985
• Protein ID: WP_001263495.1
• Coordinates: 4374522..4374920 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JG530_RS21990
• Protein ID: WP_000554757.1
• Coordinates: 4374923..4375216 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4370109..4370189	-	81	NuclAT_12	-	-
-	4370109..4370189	-	81	NuclAT_12	-	-
-	4370109..4370189	-	81	NuclAT_12	-	-
-	4370109..4370189	-	81	NuclAT_12	-	-
JG530_RS21960	4370785..4371243	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JG530_RS21965	4371504..4372961	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JG530_RS21970	4373018..4373539	-	522	Protein_4303	peptide chain release factor H	-
JG530_RS21975	4373538..4373741	-	204	Protein_4304	RNA ligase RtcB family protein	-
JG530_RS21980	4374060..4374512	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JG530_RS21985	4374522..4374920	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JG530_RS21990	4374923..4375216	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JG530_RS21995	4375268..4376323	-	1056	WP_001226188.1	DNA polymerase IV	-
JG530_RS22000	4376394..4377179	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JG530_RS22005	4377151..4378863	+	1713	Protein_4310	flagellar biosynthesis protein FlhA	-
JG530_RS22010	4379008..4379505	-	498	Protein_4311	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T184953 WP_001263495.1 NZ_CP066756:c4374920-4374522 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T184953 NZ_CP087851:1169394-1169636 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT184953 WP_000554757.1 NZ_CP066756:c4375216-4374923 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT184953 NZ_CP087851:1169626-1169883 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6