Detailed information of TA system TA184917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4472993..4473687
• Replicon: chromosome
• Accession: NZ_CP066753
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JG531_RS22575
• Protein ID: WP_001263495.1
• Coordinates: 4472993..4473391 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JG531_RS22580
• Protein ID: WP_000554757.1
• Coordinates: 4473394..4473687 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4468580..4468660	-	81	NuclAT_12	-	-
-	4468580..4468660	-	81	NuclAT_12	-	-
-	4468580..4468660	-	81	NuclAT_12	-	-
-	4468580..4468660	-	81	NuclAT_12	-	-
JG531_RS22550	4469256..4469714	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JG531_RS22555	4469975..4471432	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JG531_RS22560	4471489..4472010	-	522	Protein_4415	peptide chain release factor H	-
JG531_RS22565	4472009..4472212	-	204	Protein_4416	RNA ligase RtcB family protein	-
JG531_RS22570	4472531..4472983	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JG531_RS22575	4472993..4473391	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JG531_RS22580	4473394..4473687	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JG531_RS22585	4473739..4474794	-	1056	WP_001226186.1	DNA polymerase IV	-
JG531_RS22590	4474865..4475650	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JG531_RS22595	4475622..4477334	+	1713	Protein_4422	flagellar biosynthesis protein FlhA	-
JG531_RS22600	4477479..4477976	-	498	Protein_4423	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T184917 WP_001263495.1 NZ_CP066753:c4473391-4472993 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T184917 NZ_CP087843:c2011707-2011450 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT184917 WP_000554757.1 NZ_CP066753:c4473687-4473394 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT184917 NZ_CP087843:c2011939-2011697 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6