Detailed information of TA system TA184908

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2716192..2716563
• Replicon: chromosome
• Accession: NZ_CP066753
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: JG531_RS13735
• Protein ID: WP_042853000.1
• Coordinates: 2716369..2716563 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2716192..2716370 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG531_RS13710	2712147..2713520	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
JG531_RS13715	2713649..2714584	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JG531_RS13720	2714636..2715871	-	1236	WP_000040845.1	site-specific integrase	-
JG531_RS13725	2715873..2716088	-	216	WP_000079604.1	excisionase XisR	-
JG531_RS13730	2716188..2716376	-	189	WP_001356607.1	DUF1187 family protein	-
-	2716192..2716370	+	179	NuclAT_0	-	Antitoxin
-	2716192..2716370	+	179	NuclAT_0	-	Antitoxin
-	2716192..2716370	+	179	NuclAT_0	-	Antitoxin
-	2716192..2716370	+	179	NuclAT_0	-	Antitoxin
JG531_RS13735	2716369..2716563	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JG531_RS13740	2716620..2717429	-	810	WP_000166315.1	recombination protein RecT	-
JG531_RS13745	2717422..2720091	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
JG531_RS13750	2720172..2720348	-	177	WP_000245534.1	hypothetical protein	-
JG531_RS13755	2720342..2720563	-	222	WP_000560226.1	killing protein KilR	-
JG531_RS13760	2720610..2721458	-	849	WP_000887681.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2714636..2761257	46621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T184908 WP_042853000.1 NZ_CP066753:c2716563-2716369 [Escherichia coli O157:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T184908 NZ_CP087840:c1567130-1566888 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT184908 NZ_CP066753:2716192-2716370 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin