Detailed information of TA system TA184895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 669825..670624
• Replicon: chromosome
• Accession: NZ_CP066753
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JG531_RS03315
• Protein ID: WP_000347273.1
• Coordinates: 669825..670289 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JG531_RS03320
• Protein ID: WP_001302819.1
• Coordinates: 670289..670624 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG531_RS03285	664826..665260	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JG531_RS03290	665278..666156	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JG531_RS03295	666146..666925	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JG531_RS03300	666936..667409	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JG531_RS03305	667432..668712	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JG531_RS03310	668961..669770	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JG531_RS03315	669825..670289	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JG531_RS03320	670289..670624	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JG531_RS03325	670773..672344	-	1572	WP_001273776.1	galactarate dehydratase	-
JG531_RS03330	672719..674053	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JG531_RS03335	674069..674839	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T184895 WP_000347273.1 NZ_CP066753:c670289-669825 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T184895 NZ_CP087837:c1566602-1566345 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT184895 WP_001302819.1 NZ_CP066753:c670624-670289 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT184895 NZ_CP087837:c1566834-1566592 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Structures

Toxin

• AlphaFold DB: V0SSH7