Detailed information of TA system TA184884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23541..23805
• Replicon: plasmid unnamed1
• Accession: NZ_CP066749
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: JG532_RS27625
• Protein ID: WP_001303307.1
• Coordinates: 23541..23693 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	23748..23805 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS27600	18818..20986	+	2169	WP_000698357.1	DotA/TraY family protein	-
JG532_RS27605	21060..21710	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
JG532_RS27610	21782..21991	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
JG532_RS27615	22383..22559	+	177	WP_001054897.1	hypothetical protein	-
JG532_RS27620	23218..23469	+	252	WP_001291965.1	hypothetical protein	-
JG532_RS27625	23541..23693	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	23748..23805	+	58	NuclAT_0	-	Antitoxin
-	23748..23805	+	58	NuclAT_0	-	Antitoxin
-	23748..23805	+	58	NuclAT_0	-	Antitoxin
-	23748..23805	+	58	NuclAT_0	-	Antitoxin
JG532_RS27630	23985..25193	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
JG532_RS27635	25212..26282	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
JG532_RS27640	26275..28566	+	2292	WP_001289282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..95679	95679

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T184884 WP_001303307.1 NZ_CP066749:c23693-23541 [Escherichia coli O157:H7]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T184884 NZ_CP087830:2208788-2209048 [Moraxella bovis]
ATTGAGGCCTTATTTGTAGCATTAGGGGCGGACATCTCTGAGCGAGCAGGTTCACGCATGGCTGTGGCGCTAAACGGGCA
AGTCATGGTTTTTCATCGCCCACACCCAAGCCCAAATACTGACAAAAGTGCGGTTATGAGCATTAGAAAATGGCTTGATG
GCATGGGTATCACACCATTAGATGACGACAGCCAAACAGACGAATACGCAGACAAACAAACCGACGAACAGGAGCAGACT
CATGAACACAATGACAATTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT184884 NZ_CP066749:23748-23805 [Escherichia coli O157:H7]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin