Detailed information of TA system TA184877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4436131..4436825
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JG532_RS22520
• Protein ID: WP_001263495.1
• Coordinates: 4436131..4436529 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JG532_RS22525
• Protein ID: WP_000554757.1
• Coordinates: 4436532..4436825 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4431718..4431798	-	81	NuclAT_11	-	-
-	4431718..4431798	-	81	NuclAT_11	-	-
-	4431718..4431798	-	81	NuclAT_11	-	-
-	4431718..4431798	-	81	NuclAT_11	-	-
JG532_RS22495	4432394..4432852	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JG532_RS22500	4433113..4434570	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JG532_RS22505	4434627..4435148	-	522	Protein_4410	peptide chain release factor H	-
JG532_RS22510	4435147..4435350	-	204	Protein_4411	RNA ligase RtcB family protein	-
JG532_RS22515	4435669..4436121	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JG532_RS22520	4436131..4436529	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JG532_RS22525	4436532..4436825	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JG532_RS22530	4436877..4437932	-	1056	WP_001226186.1	DNA polymerase IV	-
JG532_RS22535	4438003..4438788	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JG532_RS22540	4438760..4440472	+	1713	Protein_4417	flagellar biosynthesis protein FlhA	-
JG532_RS22545	4440617..4441114	-	498	Protein_4418	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	4371654..4457605	85951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T184877 WP_001263495.1 NZ_CP066748:c4436529-4436131 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T184877 NZ_CP087828:c2269330-2269142 [Moraxella bovis]
ATGCCCCCAAGTAGCGATGAGATGATAAAGCGAATTACCGAAGACGGTTGGTATTTTGTAAAACAAAAAGGCTCTCACAA
GCACTACAAACACCCAACCAAAGAAGGTAAAGTAACAATCCCAAACGGTCGTGAAAACCTACCCAAAGGAACGCTAAAAA
GCATCATCAAACAAGCAGGGTTGGATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT184877 WP_000554757.1 NZ_CP066748:c4436825-4436532 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT184877 NZ_CP087828:c2269079-2268672 [Moraxella bovis]
ATGTTATTTCCCATTGCCATTGAAAAACCTGCCAATGAAAACGAATGTTATGGCGTGATTGTGCCAGACATTGCAGGGTG
TTTTAGTGCAGGCGATACACCTGATGAAGCCATTAAGAATGCTTATGAAGCCATCAGTTTGCATTTGGAAGGCATGGCAG
AAGATGGCGATACACTACCTACCGCCAAGCCCATTTTTTATCATCAAGACAATAGCGAATTTGACGGTATGTTGTGGGCA
GTGGTGGACGTGGATATGTCTGCCTTTATGGGTAAGGCGGAGAAAATCAACGTTACCTTGCCAAGCCTACTCATCACCAA
AATTGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATT
TGGGGTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6