Detailed information of TA system TA184871

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3446430..3446655
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: JG532_RS17770
• Protein ID: WP_000813263.1
• Coordinates: 3446430..3446585 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3446597..3446655 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS17735	3441884..3442597	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
JG532_RS17740	3442735..3442931	-	197	Protein_3474	TrmB family transcriptional regulator	-
JG532_RS17745	3443218..3444036	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
JG532_RS17750	3444188..3444559	-	372	WP_000090264.1	antitermination protein	-
JG532_RS17755	3444549..3444920	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
JG532_RS17760	3444933..3445982	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
JG532_RS17765	3445984..3446262	-	279	WP_001341388.1	hypothetical protein	-
JG532_RS17770	3446430..3446585	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3446597..3446655	+	59	-	-	Antitoxin
JG532_RS17775	3447190..3447963	+	774	WP_000160654.1	alpha/beta hydrolase	-
JG532_RS17780	3448315..3448728	-	414	WP_001151233.1	DUF977 family protein	-
JG532_RS17785	3448744..3449514	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JG532_RS17790	3449536..3450282	-	747	WP_000788751.1	ATP-binding protein	-
JG532_RS17795	3450289..3451380	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T184871 WP_000813263.1 NZ_CP066748:c3446585-3446430 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T184871 NZ_CP087828:c1570549-1570292 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTAATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT184871 NZ_CP066748:3446597-3446655 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin