Detailed information of TA system TA184759

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 17357..17562
• Replicon: plasmid p148_55kb
• Accession: NZ_CP066647
• Organism: Enterococcus faecium strain Dallas 148

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP76_RS14000
• Protein ID: WP_002295624.1
• Coordinates: 17357..17479 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	17461..17562 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP76_RS13960	12433..12636	-	204	WP_002300097.1	hypothetical protein	-
JCP76_RS13965	12796..13371	-	576	WP_002300096.1	recombinase family protein	-
JCP76_RS13970	13387..13992	-	606	WP_002300095.1	Fic family protein	-
JCP76_RS13975	14598..14777	-	180	WP_002305204.1	hypothetical protein	-
JCP76_RS13980	15158..15865	-	708	WP_002305202.1	hypothetical protein	-
JCP76_RS13985	15858..16295	-	438	WP_002287515.1	hypothetical protein	-
JCP76_RS13990	16300..16500	-	201	WP_002305199.1	hypothetical protein	-
JCP76_RS13995	16943..17203	-	261	WP_049055261.1	hypothetical protein	-
JCP76_RS14000	17357..17479	+	123	WP_002295624.1	putative holin-like toxin	Toxin
-	17461..17562	-	102	NuclAT_0	-	Antitoxin
JCP76_RS14005	17639..17896	-	258	WP_002305195.1	hypothetical protein	-
JCP76_RS14010	17918..18403	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP76_RS14015	18406..19305	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP76_RS14020	19321..19914	-	594	WP_002287520.1	transcriptional regulator	-
JCP76_RS14025	19988..20137	-	150	WP_002295632.1	hypothetical protein	-
JCP76_RS14030	20153..22357	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55497	55497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184759 WP_002295624.1 NZ_CP066647:17357-17479 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184759 NZ_CP087800:1423372-1423554 [Moraxella bovis]
ATGAAGTATTCAGAGTTTCAAAGGTGGCTATTGGCACAAGGGGCAACGGTAAACAAGCAAGGCGGTAAAGGCTCGCACCG
TAAAGTTACCCTAAATGGCAAAACCACCACTTTCCCTTATCACGGCAGTAAAGAGATTGGGGAAGGACTAAGAAAGAAAA
TTTTAAAAGACTTGGATTTATAG

Antitoxin

Nucleotide

• Length: 102 bp

>AT184759 NZ_CP066647:c17562-17461 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin