Detailed information of TA system TA184753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 28588..28793
• Replicon: plasmid p144_1_56kb
• Accession: NZ_CP066640
• Organism: Enterococcus faecium strain Dallas 144_1

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP95_RS15445
• Protein ID: WP_002295624.1
• Coordinates: 28588..28710 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	28692..28793 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP95_RS15405	23664..23867	-	204	WP_002300097.1	hypothetical protein	-
JCP95_RS15410	24027..24602	-	576	WP_002300096.1	recombinase family protein	-
JCP95_RS15415	24618..25223	-	606	WP_002300095.1	Fic family protein	-
JCP95_RS15420	25829..26008	-	180	WP_002305204.1	hypothetical protein	-
JCP95_RS15425	26389..27096	-	708	WP_002305202.1	hypothetical protein	-
JCP95_RS15430	27089..27526	-	438	WP_002287515.1	hypothetical protein	-
JCP95_RS15435	27531..27731	-	201	WP_002305199.1	hypothetical protein	-
JCP95_RS15440	28174..28434	-	261	WP_049055261.1	hypothetical protein	-
JCP95_RS15445	28588..28710	+	123	WP_002295624.1	putative holin-like toxin	Toxin
-	28692..28793	-	102	NuclAT_0	-	Antitoxin
JCP95_RS15450	28870..29127	-	258	WP_002305195.1	hypothetical protein	-
JCP95_RS15455	29149..29634	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP95_RS15460	29637..30536	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP95_RS15465	30552..31145	-	594	WP_002287520.1	transcriptional regulator	-
JCP95_RS15470	31219..31368	-	150	WP_002295632.1	hypothetical protein	-
JCP95_RS15475	31384..33588	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56412	56412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184753 WP_002295624.1 NZ_CP066640:28588-28710 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184753 NZ_CP087798:c2132850-2132449 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184753 NZ_CP066640:c28793-28692 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin