Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 137675..138210 Replicon plasmid p100_1_156kb
Accession NZ_CP066577
Organism Enterococcus faecium strain Dallas 100_1

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag JCP75_RS15220 Protein ID WP_002305108.1
Coordinates 137675..137947 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag JCP75_RS15225 Protein ID WP_002303154.1
Coordinates 137947..138210 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP75_RS15190 132685..133872 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
JCP75_RS15195 134060..135007 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
JCP75_RS15200 135300..135848 - 549 WP_002292681.1 hypothetical protein -
JCP75_RS15205 135849..136706 - 858 WP_002292680.1 AAA family ATPase -
JCP75_RS15210 136992..137279 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
JCP75_RS15215 137269..137598 - 330 WP_002292678.1 AbrB family transcriptional regulator -
JCP75_RS15220 137675..137947 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
JCP75_RS15225 137947..138210 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JCP75_RS15230 138316..138732 - 417 WP_002303153.1 hypothetical protein -
JCP75_RS15235 138738..139301 - 564 Protein_147 IS6 family transposase -
JCP75_RS15240 139381..140342 + 962 Protein_148 IS3 family transposase -
JCP75_RS15245 140398..141078 - 681 WP_002319817.1 IS6-like element ISS1N family transposase -
JCP75_RS15250 141607..142797 - 1191 WP_002314359.1 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..156278 156278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T184710 WP_002305108.1 NZ_CP066577:c137947-137675 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T184710 NZ_CP087784:c2251451-2251263 [Moraxella bovis]
ATGCCCCCAAGTAGCGATGAGATGATAAAGCGAATTACCGAAGACGGTTGGTATTTTGTAAAACAAAAAGGCTCTCACAA
GCACTACAAACACCCAACCAAAGAAGGTAAAGTAACAATCCCAAACGGTCGTGAAAACCTACCCAAAGGAACGCTAAAAA
GCATCATCAAACAAGCAGGGTTGGATTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT184710 WP_002303154.1 NZ_CP066577:c138210-137947 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT184710 NZ_CP087784:c2251200-2250793 [Moraxella bovis]
ATGTTATTTCCCATTGCCATTGAAAAACCTGCCAATGAAAACGAATGTTATGGCGTGATTGTGCCAGACATTGCAGGGTG
TTTTAGTGCAGGCGATACACCTGATGAAGCCATTAAGAATGCTTATGAAGCCATCAGTTTGCATTTGGAAGGCATGGCAG
AAGATGGCGATACACTACCTACCGCCAAGCCCATTTTTTATCATCAAGACAATAGCGAATTTGACGGTATGTTGTGGGCA
GTGGTGGACGTGGATATGTCTGCCTTTATGGGTAAGGCGGAGAAAATCAACGTTACCTTGCCAAGCCTACTCATCACCAA
AATTGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATT
TGGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References