Detailed information of TA system TA184650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 474998..475722
• Replicon: chromosome
• Accession: NZ_CP066552
• Organism: Pectobacterium carotovorum strain 2A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: JFY74_RS02150
• Protein ID: WP_039482109.1
• Coordinates: 474998..475309 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFY74_RS02155
• Protein ID: WP_198997989.1
• Coordinates: 475306..475722 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY74_RS02130	470642..471376	+	735	WP_039512783.1	type I-E CRISPR-associated protein Cas5/CasD	-
JFY74_RS02135	471376..472023	+	648	WP_198999024.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
JFY74_RS02140	472051..472968	+	918	WP_010308688.1	type I-E CRISPR-associated endonuclease Cas1	-
JFY74_RS02145	472969..473262	+	294	WP_102117148.1	type I-E CRISPR-associated endoribonuclease Cas2	-
JFY74_RS02150	474998..475309	+	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
JFY74_RS02155	475306..475722	+	417	WP_198997989.1	helix-turn-helix domain-containing protein	Antitoxin
JFY74_RS02160	475945..476448	-	504	WP_198997990.1	hypothetical protein	-
JFY74_RS02165	476736..477026	+	291	Protein_413	type I-E CRISPR-associated endoribonuclease Cas2	-
JFY74_RS02170	478727..479185	+	459	WP_039491727.1	hypothetical protein	-
JFY74_RS02175	479283..480635	-	1353	WP_198997991.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T184650 WP_039482109.1 NZ_CP066552:474998-475309 [Pectobacterium carotovorum]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T184650 NZ_CP087719:5123795-5124199 [Roseomonas sp. OT10]
GTGCGCTACATGCTGGATACCAACATCGTCAGCGACATCATCCGGAACCCGTTGGGGGCTGCCGCGCAGCGTGCGCGCGC
CGAGGGGGATGCAGTGTGCGTCAGCGTCATCGTCGCGGCGGAGCTTCGCTATGGCTGCGCCCGCAAGGGTTCGCCGAAGC
TGACGCGCCGCGTCGCGCAGTTCCTGGCGGAGGTGCCGGTGCTTCCGTTCGACGCTCCGGCGGATGAAGCTTACGGCACG
CTACGGGCGGAGTTGGAAGCGGCAGGGAGGCCGATTGGGGGGAATGATCTGCTGATCGCCGCTCATGCTAGTGCGTTGGG
AGCTACAGTGGTGACAGCGAATCTGGGAGAGTTTGTGCGGGTCCCAGGATTGAATGCGGAGAATTGGATGGAGACCTCGG
GATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15431.69 Da
• Isoelectric Point: 4.5390

>AT184650 WP_198997989.1 NZ_CP066552:475306-475722 [Pectobacterium carotovorum]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRILMDQYQLTQSDFEAEIGKKSLVSRILNGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT184650 NZ_CP087719:5123544-5123792 [Roseomonas sp. OT10]
ATGCCCTCGCCTGCCCCAGCCTCGCGTCAGGTCCGCCTGTTCCGCAACAATCGCAATCAGGCCATCCGCATCCCGGTGGA
GTTCGAGCTTCCGGGGGAGGAGGCGATCATCACCCGCGATGGCGACCGGTTGATCATCGAGCCGGTGCGCCGCCGGGGAC
TCGCCGCCCTGCTGGATAGCTGGGAGCCGCTGGACGAGGAGCTTCCGGAGCCCGAGGACCTGCCCCTGGCGCCGAAGGAT
GTCTTCTGA

Structures

Toxin

• AlphaFold DB: A0A093VC08