Detailed information of TA system TA184642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1411184..1411878
• Replicon: chromosome
• Accession: NZ_CP066546
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JE032_RS06540
• Protein ID: WP_009046630.1
• Coordinates: 1411184..1411480 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A554PLA7
• Locus tag: JE032_RS06545
• Protein ID: WP_009046631.1
• Coordinates: 1411477..1411878 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE032_RS06505	1406718..1407263	-	546	WP_009046626.1	membrane protein	-
JE032_RS06510	1407260..1407514	-	255	WP_007926835.1	acyl carrier protein	-
JE032_RS06515	1407524..1407784	-	261	WP_007926834.1	acyl carrier protein	-
JE032_RS06520	1407759..1408574	-	816	WP_016704468.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
JE032_RS06525	1408550..1409281	-	732	WP_041985527.1	beta-ketoacyl synthase chain length factor	-
JE032_RS06530	1409615..1410385	+	771	WP_007926828.1	ParA family protein	-
JE032_RS06535	1410511..1410945	-	435	WP_016704469.1	thioredoxin TrxC	-
JE032_RS06540	1411184..1411480	+	297	WP_009046630.1	type II toxin-antitoxin system HigB family toxin	Toxin
JE032_RS06545	1411477..1411878	+	402	WP_009046631.1	transcriptional regulator	Antitoxin
JE032_RS06550	1411882..1413345	-	1464	WP_016704471.1	YdiU family protein	-
JE032_RS06555	1413394..1416744	-	3351	WP_009046633.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (15-133)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11489.27 Da
• Isoelectric Point: 10.5069

>T184642 WP_009046630.1 NZ_CP066546:1411184-1411480 [Pseudomonas chlororaphis subsp. aureofaciens]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGAFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETG

Nucleotide

• Length: 297 bp

>T184642 NZ_CP087716:c1229249-1228968 [Acinetobacter pittii]
ATGAGCTATAACTTAGACTTTTTAGAAGAAGCATTAGAAGAATGGAATAAGCTTAATCCTAGTATTAAGCAACCAATGAA
AAAGAAACTGATGAAAGTCTTAGAGAATCCTAGAATCCCTAAGAATAAGCTATCAGGGCATCCTAACCGATATAAAGTCA
AACTTCGTAGTGTTGGCTATCGTTTGGTTTATGAGGTCATAGATGATGAGGTTGTTGTATTAGTAATTGCTGTAGGCAAA
CGTGAAAATAATGCTGTCTACAATACGGCAAGCTCACGATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14840.85 Da
• Isoelectric Point: 4.4394

>AT184642 WP_009046631.1 NZ_CP066546:1411477-1411878 [Pseudomonas chlororaphis subsp. aureofaciens]
MNVLVKQAADHWHFVAPVLRKPNTEADYDALVAALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT184642 NZ_CP087716:c1229487-1229239 [Acinetobacter pittii]
ATGACTCAACTAATCCATGCACGAATGACTGCAAGTATTACTGAACTCAAAAAGAGTCCTATGGATACTGTATTAGCAGG
TAAAGGTGAAGCTGTTGCTATTCTTAACCGTAATACCCCTGCTTTCTACTGTATTCCTGCTGATCTATATGAAAATATGA
TTGAACAGCTAGAGGACTTAGAACTCAATAAAATTGCAGATACTCGTGCGAATCAAAAGCGTATTCGAGTAAACATAAAT
GAGCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A554PLA7