Detailed information of TA system TA184635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19120..19373
• Replicon: plasmid pBTS_02
• Accession: NZ_CP066544
• Organism: Klebsiella pneumoniae strain BTS

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: D6T75_RS27380
• Protein ID: WP_001312851.1
• Coordinates: 19120..19269 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19314..19373 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T75_RS27350	14571..15020	-	450	Protein_20	PAS domain-containing protein	-
D6T75_RS27355	15100..15804	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
D6T75_RS27360	15851..16480	-	630	WP_200303249.1	CPBP family intramembrane metalloprotease	-
D6T75_RS27365	17419..18276	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
D6T75_RS27370	18269..18343	-	75	WP_001375168.1	RepA leader peptide Tap	-
D6T75_RS27375	18588..18836	-	249	WP_000083837.1	replication regulatory protein RepA	-
D6T75_RS27380	19120..19269	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	19314..19373	+	60	NuclAT_0	-	Antitoxin
-	19314..19373	+	60	NuclAT_0	-	Antitoxin
-	19314..19373	+	60	NuclAT_0	-	Antitoxin
-	19314..19373	+	60	NuclAT_0	-	Antitoxin
D6T75_RS27385	19574..19804	-	231	WP_001736714.1	hypothetical protein	-
D6T75_RS27390	19968..20567	-	600	WP_032083981.1	hypothetical protein	-
D6T75_RS27395	20953..21153	-	201	WP_015059022.1	hypothetical protein	-
D6T75_RS27400	21285..21845	-	561	WP_000139328.1	fertility inhibition protein FinO	-
D6T75_RS27405	21900..22646	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
D6T75_RS27410	22666..22827	-	162	Protein_32	DNA helicase	-
D6T75_RS27415	22891..23595	+	705	WP_101429027.1	IS6-like element IS26 family transposase	-
D6T75_RS27420	23648..23713	+	66	Protein_34	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaCTX-M-65	-	1..88050	88050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184635 WP_001312851.1 NZ_CP066544:c19269-19120 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184635 NZ_CP087707:8429-8911 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184635 NZ_CP066544:19314-19373 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin