Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4698499..4699145 Replicon chromosome
Accession NZ_CP066543
Organism Klebsiella pneumoniae strain BTS

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag D6T75_RS24230 Protein ID WP_002920560.1
Coordinates 4698798..4699145 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag D6T75_RS24225 Protein ID WP_002920557.1
Coordinates 4698499..4698798 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T75_RS24205 4694705..4695463 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
D6T75_RS24210 4695513..4696343 - 831 WP_200302565.1 rhomboid family intramembrane serine protease GlpG -
D6T75_RS24215 4696394..4696723 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
D6T75_RS24220 4696928..4698436 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
D6T75_RS24225 4698499..4698798 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
D6T75_RS24230 4698798..4699145 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D6T75_RS24235 4699321..4701768 - 2448 WP_200302567.1 glycogen phosphorylase -
D6T75_RS24240 4701786..4703219 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T184632 WP_002920560.1 NZ_CP066543:c4699145-4698798 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T184632 NZ_CP087706:c4724748-4724464 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT184632 WP_002920557.1 NZ_CP066543:c4698798-4698499 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT184632 NZ_CP087706:c4724980-4724738 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References