Detailed information of TA system TA184616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 89683..90340
• Replicon: plasmid pBTS_01
• Accession: NZ_CP066542
• Organism: Klebsiella pneumoniae strain BTS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: D6T75_RS00555
• Protein ID: WP_000270043.1
• Coordinates: 89683..90033 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6T75_RS00560
• Protein ID: WP_000124640.1
• Coordinates: 90038..90340 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T75_RS00520	86157..86654	-	498	WP_000062185.1	hypothetical protein	-
D6T75_RS00525	86657..87145	-	489	WP_200302253.1	DUF1643 domain-containing protein	-
D6T75_RS00530	87242..87577	+	336	WP_000683476.1	hypothetical protein	-
D6T75_RS00535	87592..88062	-	471	WP_001281821.1	hypothetical protein	-
D6T75_RS00540	88055..88426	-	372	WP_000516916.1	hypothetical protein	-
D6T75_RS00545	88437..88631	-	195	WP_000343597.1	hypothetical protein	-
D6T75_RS00550	88972..89520	-	549	WP_001061574.1	transcriptional regulator	-
D6T75_RS00555	89683..90033	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6T75_RS00560	90038..90340	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
D6T75_RS00565	90367..90660	-	294	WP_001239997.1	chromosome segregation protein ParM	-
D6T75_RS00570	90748..91020	-	273	WP_001043047.1	HU family DNA-binding protein	-
D6T75_RS00575	91078..91605	-	528	WP_001236377.1	thermonuclease family protein	-
D6T75_RS00580	91836..92693	-	858	WP_001167032.1	hypothetical protein	-
D6T75_RS00585	92680..92910	-	231	WP_000972663.1	hypothetical protein	-
D6T75_RS00590	92910..93428	-	519	WP_000210756.1	nitrite reductase	-
D6T75_RS00595	93425..93871	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
D6T75_RS00600	93871..94230	-	360	WP_000422768.1	hypothetical protein	-
D6T75_RS00605	94287..94715	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA12 / aadA2 / qacE / floR / tet(G) / rmtB / blaTEM-2 / erm(42) / blaCMY-2 / catB3 / blaOXA-10 / aph(3')-Ia / dfrA14 / ARR-3 / cmlA1 / ant(3'')-Ia / sul1 / blaDHA-1	-	1..188594	188594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T184616 WP_000270043.1 NZ_CP066542:89683-90033 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T184616 NZ_CP087696:c1858857-1858654 [Xylella taiwanensis]
ATGACGTTGCCGCTTCACTGGCGTACATCCGCACGCGATGACTTGGCCTCGTTGATACGTTTCATCGCTTACGAAAATCC
GCTTACCGCGCGGCGCATGAAAGTACTCATTGAAGCGTCCGTGTTACTAGCCTCCGCACATCCCTACATGTTCCGACCTG
GCCGTGTACCAGGGACACGAGAGATCGTGGCCCACCCGAACTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT184616 WP_000124640.1 NZ_CP066542:90038-90340 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT184616 NZ_CP087696:c1859048-1858854 [Xylella taiwanensis]
ATGAAAACCACGCCGCTCGACCCGATCATTTCCGAGTTCGCCACCGAAGAACAGGCGGCCAGCTACGACCGTTGGTTCCG
CGCCAAGGTCCAGGCAAGCCTGGACGATTCGCGCCCAAGCGTTCCACACGATCAAGTGATGGCTGAGATGGATGCGATCA
TCGCTGAAGCCGAGCAGCATCGTAAGCAAACATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9