Detailed information of TA system TA184613

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 153632..153885
• Replicon: plasmid pKPC2_090374
• Accession: NZ_CP066536
• Organism: Klebsiella pneumoniae strain WCHKP090374

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H3G96_RS28895
• Protein ID: WP_001312851.1
• Coordinates: 153736..153885 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	153632..153691 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G96_RS28855	149292..149357	-	66	Protein_188	helix-turn-helix domain-containing protein	-
H3G96_RS28860	149410..150114	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H3G96_RS28865	150178..150339	+	162	Protein_190	DNA helicase	-
H3G96_RS28870	150359..151105	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H3G96_RS28875	151160..151720	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H3G96_RS28880	151852..152052	+	201	WP_015059022.1	hypothetical protein	-
H3G96_RS28885	152438..153037	+	600	WP_032083981.1	hypothetical protein	-
H3G96_RS28890	153201..153431	+	231	WP_001736714.1	hypothetical protein	-
-	153632..153691	-	60	NuclAT_1	-	Antitoxin
-	153632..153691	-	60	NuclAT_1	-	Antitoxin
-	153632..153691	-	60	NuclAT_1	-	Antitoxin
-	153632..153691	-	60	NuclAT_1	-	Antitoxin
H3G96_RS28895	153736..153885	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H3G96_RS28900	154169..154417	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154728	154728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184613 WP_001312851.1 NZ_CP066536:153736-153885 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184613 NZ_CP087696:c31057-30653 [Xylella taiwanensis]
TTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGAAAAGGTCGATCC
AAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGACCAAGTTGCTTG
CGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATATGGTCGCATTCGT
GCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGTACTGGGGATGAC
ATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAATGTGCATCCTCGG
TTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT184613 NZ_CP066536:c153691-153632 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin