Detailed information of TA system TA184608

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40472..40898
• Replicon: plasmid pKPC2_090374
• Accession: NZ_CP066536
• Organism: Klebsiella pneumoniae strain WCHKP090374

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H3G96_RS28175
• Protein ID: WP_001312861.1
• Coordinates: 40740..40898 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40472..40696 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G96_RS28150	36339..36866	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H3G96_RS28155	36924..37157	+	234	WP_000006003.1	DUF905 family protein	-
H3G96_RS28160	37218..39241	+	2024	Protein_49	ParB/RepB/Spo0J family partition protein	-
H3G96_RS28165	39310..39744	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H3G96_RS28170	39741..40460	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	40472..40696	+	225	NuclAT_0	-	Antitoxin
-	40472..40696	+	225	NuclAT_0	-	Antitoxin
-	40472..40696	+	225	NuclAT_0	-	Antitoxin
-	40472..40696	+	225	NuclAT_0	-	Antitoxin
H3G96_RS28175	40740..40898	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H3G96_RS28180	41136..41513	-	378	Protein_53	hypothetical protein	-
H3G96_RS28185	41813..42109	+	297	WP_001272251.1	hypothetical protein	-
H3G96_RS28190	42220..43041	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H3G96_RS28195	43338..43985	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H3G96_RS28200	44262..44645	+	384	WP_000124981.1	relaxosome protein TraM	-
H3G96_RS28205	44836..45522	+	687	WP_015059009.1	PAS domain-containing protein	-
H3G96_RS28210	45616..45843	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154728	154728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184608 WP_001312861.1 NZ_CP066536:40740-40898 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184608 NZ_CP087695:1369398-1369670 [Xylella taiwanensis]
ATGCGCCGTCCTGCTTATTCTTCGCAGTTCAAGCGTGATGTGCGAGTGTCTCAGAAGCGCGGTAAGGACATAGAGAAACT
TAAGATTCTAATAACTTTGCTGATTGAGGGTAGTCCGTTGCCTGCGATGTATTTGGATCATCCGCTTAAGGGGCAGTGGC
GTAGTTTCCGGGATGCTCATGTTGAGCCGGATTGGTTGTTAATCTATAAGGTTGATGGTGATGTGGTGCGCTTTGAGCGT
ACTGGTCGTCATGCTGATTTGTTCGACGATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT184608 NZ_CP066536:40472-40696 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin