Detailed information of TA system TA184583

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104898..105151
• Replicon: plasmid pKPC2_090361
• Accession: NZ_CP066529
• Organism: Klebsiella pneumoniae strain WCHKP090361

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H3G66_RS27155
• Protein ID: WP_001312851.1
• Coordinates: 105002..105151 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	104898..104957 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G66_RS27115	100558..100623	-	66	Protein_132	helix-turn-helix domain-containing protein	-
H3G66_RS27120	100676..101380	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H3G66_RS27125	101444..101605	+	162	Protein_134	DNA helicase	-
H3G66_RS27130	101625..102371	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H3G66_RS27135	102426..102986	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H3G66_RS27140	103118..103318	+	201	WP_015059022.1	hypothetical protein	-
H3G66_RS27145	103704..104303	+	600	WP_032083981.1	hypothetical protein	-
H3G66_RS27150	104467..104697	+	231	WP_001736714.1	hypothetical protein	-
-	104898..104957	-	60	NuclAT_1	-	Antitoxin
-	104898..104957	-	60	NuclAT_1	-	Antitoxin
-	104898..104957	-	60	NuclAT_1	-	Antitoxin
-	104898..104957	-	60	NuclAT_1	-	Antitoxin
H3G66_RS27155	105002..105151	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H3G66_RS27160	105435..105683	+	249	WP_000083837.1	replication regulatory protein RepA	-
H3G66_RS27165	105928..106002	+	75	WP_001375168.1	RepA leader peptide Tap	-
H3G66_RS27170	105995..106852	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
H3G66_RS27175	107791..108444	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H3G66_RS27180	108537..108794	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H3G66_RS27185	108727..109128	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H3G66_RS27190	109377..109792	+	416	Protein_147	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65 / fosA3	-	1..144228	144228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184583 WP_001312851.1 NZ_CP066529:105002-105151 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184583 NZ_CP087691:c450975-450772 [Xylella taiwanensis]
ATGACGTTGCCGCTTCACTGGCGTACATCCGCACGCGATGACTTGGCCTCGTTGATACGTTTCATCGCTTACGAAAATCC
GCTTACCGCGCGGCGCATGAAAGTACTCATTGAAGCGTCCGTGTTACTAGCCTCCGCACATCCCTACATGTTCCGACCTG
GCCGTGTACCAGGGACACGAGAGATCGTGGCCCACCCGAACTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT184583 NZ_CP066529:c104957-104898 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin