Detailed information of TA system TA184579

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47681..48107
• Replicon: plasmid pKPC2_090361
• Accession: NZ_CP066529
• Organism: Klebsiella pneumoniae strain WCHKP090361

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H3G66_RS26780
• Protein ID: WP_001312861.1
• Coordinates: 47681..47839 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47883..48107 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G66_RS26745	42725..42952	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
H3G66_RS26750	43046..43732	-	687	WP_015059009.1	PAS domain-containing protein	-
H3G66_RS26755	43923..44306	-	384	WP_000124981.1	relaxosome protein TraM	-
H3G66_RS26760	44594..45241	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H3G66_RS26765	45538..46359	-	822	WP_001234445.1	DUF945 domain-containing protein	-
H3G66_RS26770	46470..46766	-	297	WP_001272251.1	hypothetical protein	-
H3G66_RS26775	47066..47443	+	378	Protein_64	hypothetical protein	-
H3G66_RS26780	47681..47839	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	47883..48107	-	225	NuclAT_0	-	Antitoxin
-	47883..48107	-	225	NuclAT_0	-	Antitoxin
-	47883..48107	-	225	NuclAT_0	-	Antitoxin
-	47883..48107	-	225	NuclAT_0	-	Antitoxin
H3G66_RS26785	48119..48838	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
H3G66_RS26790	48835..49269	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H3G66_RS26795	49338..51361	-	2024	Protein_68	ParB/RepB/Spo0J family partition protein	-
H3G66_RS26800	51422..51655	-	234	WP_000006003.1	DUF905 family protein	-
H3G66_RS26805	51713..52240	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65 / fosA3	-	1..144228	144228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184579 WP_001312861.1 NZ_CP066529:c47839-47681 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184579 NZ_CP087690:c1257508-1257254 [Xylella taiwanensis]
ATGAAGCGTAAGCACGCTAAGACCCTCAGCACCATAGACACGCGCCCTGTTTCCGCCAACATCCAATGGCGCGATATTGA
AGCACTCTTCTTGGAGCTTGGAGCGACAGTAGAAGAACGTGAAGGGTCTCGTGTGCTGGTGCGGCTGTTCGGTGAACGCC
GTGTATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGCGCTGTTGAATCGATCCGAGCTTGGTTGAAATCGAAC
GGAGTGACACCATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT184579 NZ_CP066529:c48107-47883 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin