Detailed information of TA system TA184553

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 144783..145036
• Replicon: plasmid pKPC2_090357
• Accession: NZ_CP066524
• Organism: Klebsiella pneumoniae strain WCHKP090357

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H3G71_RS27760
• Protein ID: WP_001312851.1
• Coordinates: 144887..145036 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	144783..144842 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G71_RS27720	140443..140508	-	66	Protein_175	helix-turn-helix domain-containing protein	-
H3G71_RS27725	140561..141265	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H3G71_RS27730	141329..141490	+	162	Protein_177	DNA helicase	-
H3G71_RS27735	141510..142256	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H3G71_RS27740	142311..142871	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H3G71_RS27745	143003..143203	+	201	WP_015059022.1	hypothetical protein	-
H3G71_RS27750	143589..144188	+	600	WP_032083981.1	hypothetical protein	-
H3G71_RS27755	144352..144582	+	231	WP_001736714.1	hypothetical protein	-
-	144783..144842	-	60	NuclAT_1	-	Antitoxin
-	144783..144842	-	60	NuclAT_1	-	Antitoxin
-	144783..144842	-	60	NuclAT_1	-	Antitoxin
-	144783..144842	-	60	NuclAT_1	-	Antitoxin
H3G71_RS27760	144887..145036	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H3G71_RS27765	145320..145568	+	249	WP_000083837.1	replication regulatory protein RepA	-
H3G71_RS27770	145813..145887	+	75	WP_001375168.1	RepA leader peptide Tap	-
H3G71_RS27775	145880..146737	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
H3G71_RS27780	147676..148329	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H3G71_RS27785	148422..148679	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H3G71_RS27790	148612..149013	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65	-	1..149116	149116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184553 WP_001312851.1 NZ_CP066524:144887-145036 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184553 NZ_CP087685:8659-8982 [Xylella taiwanensis]
ATGACTCAACGCGGCGACCTTGTAACGGTTTCCCTGCAAGGCGATTATGGCAAACCTAGACCGGCGTTAATCCTCCAATC
AGACCTGCTGATGGAGCTAGATAGTGTCGTACTGTGTCCGGTCACAAGTGAGCTACGTAATGCGATATTTCGCATCACCG
TTGAACCAAACATAGCCAATGGGCTACGGACGCTCTCGCAAATCATGGTAGACAAAATTTCAACACTGCCGCGTAGCAAA
ATCAGCAAACCTTTTGGACATCTCAATGACGAGAGGATAAAAGCAGTAGAAAAAGCACTGTTGCTCATCGTTGGTATCAT
CTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184553 NZ_CP066524:c144842-144783 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin