Detailed information of TA system TA184548

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30804..31230
• Replicon: plasmid pKPC2_090357
• Accession: NZ_CP066524
• Organism: Klebsiella pneumoniae strain WCHKP090357

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H3G71_RS27035
• Protein ID: WP_001312861.1
• Coordinates: 31072..31230 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30804..31028 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G71_RS27010	26671..27198	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H3G71_RS27015	27256..27489	+	234	WP_000006003.1	DUF905 family protein	-
H3G71_RS27020	27550..29573	+	2024	Protein_35	ParB/RepB/Spo0J family partition protein	-
H3G71_RS27025	29642..30076	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H3G71_RS27030	30073..30792	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	30804..31028	+	225	NuclAT_0	-	Antitoxin
-	30804..31028	+	225	NuclAT_0	-	Antitoxin
-	30804..31028	+	225	NuclAT_0	-	Antitoxin
-	30804..31028	+	225	NuclAT_0	-	Antitoxin
H3G71_RS27035	31072..31230	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H3G71_RS27040	31468..31845	-	378	Protein_39	hypothetical protein	-
H3G71_RS27045	32145..32441	+	297	WP_001272251.1	hypothetical protein	-
H3G71_RS27050	32552..33373	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H3G71_RS27055	33670..34317	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H3G71_RS27060	34594..34977	+	384	WP_000124981.1	relaxosome protein TraM	-
H3G71_RS27065	35168..35854	+	687	WP_015059009.1	PAS domain-containing protein	-
H3G71_RS27070	35948..36175	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65	-	1..149116	149116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184548 WP_001312861.1 NZ_CP066524:31072-31230 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184548 NZ_CP087683:c1447927-1447523 [Xylella taiwanensis]
TTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGAAAAGGTCGATCC
AAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGACCAAGTTGCTTG
CGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATATGGTCGCATTCGT
GCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGTACTGGGGATGAC
ATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAATGTGCATCCTCGG
TTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT184548 NZ_CP066524:30804-31028 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin