Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 52121..52848 Replicon plasmid pLAP2_090329
Accession NZ_CP066519
Organism Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag H3G99_RS27895 Protein ID WP_004118600.1
Coordinates 52121..52432 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag H3G99_RS27900 Protein ID WP_000990392.1
Coordinates 52429..52848 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G99_RS27875 48635..49354 + 720 WP_001102107.1 (Fe-S)-binding protein -
H3G99_RS27880 49365..50792 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
H3G99_RS27885 50785..51480 + 696 WP_004118595.1 lactate utilization protein C -
H3G99_RS27890 51530..51910 - 381 Protein_48 gluconate permease -
H3G99_RS27895 52121..52432 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
H3G99_RS27900 52429..52848 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
H3G99_RS27905 52885..54087 - 1203 WP_003031541.1 hypothetical protein -
H3G99_RS27910 54080..54409 - 330 WP_004118613.1 hypothetical protein -
H3G99_RS27915 54406..55065 - 660 WP_000078540.1 hypothetical protein -
H3G99_RS27920 55489..56082 - 594 WP_044348674.1 hypothetical protein -
H3G99_RS27925 56398..56751 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
H3G99_RS27930 56799..57161 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / blaLAP-2 / qnrS1 / sul1 / qacE / dfrA1 / tet(A) - 1..228076 228076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T184528 WP_004118600.1 NZ_CP066519:52121-52432 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T184528 NZ_CP087678:2274809-2275087 [Xylella taiwanensis]
ATGATTAAAACCTTTGCCGACCAGGACACGGAATCTTTGTTCAACGGGTCACGGGTACGTCGCTTTATTCACATAGAAGC
GGTCGCACGCCGGAAGCTGATTCAATTGCATGCAGCAGCGACCTTGAGCTTTCTCAATGCGCCGCCTGGTAATCGGCTTG
AAACCTTAAAAGGGGACCGCCAAGGGCAGCACAGCATCCGTATCAACAATCAATTCCGCCTCTGTTTCATTTGGGAAAAC
GGCGATGCCTATCAGGTTGAAATTGTCGATTATCACTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT184528 WP_000990392.1 NZ_CP066519:52429-52848 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT184528 NZ_CP087678:2275091-2275390 [Xylella taiwanensis]
ATGAGCATGTCCAAGACACTTCCCCCCATTCACCCAGGAGAAATTCTCCGGGAAGAATTTATGGCCCCTCTAGGCTTATC
CAGCAATGCGCTGGCCAAGGCGATTGGCGTGACGGCGGCACGGATCAACGAGATTGTGCGCGAACGACGTGGGATCACGG
CGGATACTGCACTGCGTCTGGCACGTTACTTTGGCACCGATCCGCAAAGCTGGCTGAATCTGGAACAGCACTATGCACTC
GAATGCGCCAAACGCGACCTTGGAGAGGCCCTGTCACGTATTCACCCTCGCGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References