Detailed information of TA system TA184520

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52800..53226
• Replicon: plasmid pKPC2_090329
• Accession: NZ_CP066518
• Organism: Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H3G99_RS27000
• Protein ID: WP_001312861.1
• Coordinates: 52800..52958 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53002..53226 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G99_RS26965	47855..48082	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
H3G99_RS26970	48176..48862	-	687	WP_015059009.1	PAS domain-containing protein	-
H3G99_RS26975	49053..49436	-	384	WP_000124981.1	relaxosome protein TraM	-
H3G99_RS26980	49713..50360	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H3G99_RS26985	50657..51478	-	822	WP_001234445.1	DUF945 domain-containing protein	-
H3G99_RS26990	51589..51885	-	297	WP_001272251.1	hypothetical protein	-
H3G99_RS26995	52185..52562	+	378	Protein_69	hypothetical protein	-
H3G99_RS27000	52800..52958	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	53002..53226	-	225	NuclAT_0	-	Antitoxin
-	53002..53226	-	225	NuclAT_0	-	Antitoxin
-	53002..53226	-	225	NuclAT_0	-	Antitoxin
-	53002..53226	-	225	NuclAT_0	-	Antitoxin
H3G99_RS27005	53238..53957	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
H3G99_RS27010	53954..54388	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H3G99_RS27015	54457..56480	-	2024	Protein_73	ParB/RepB/Spo0J family partition protein	-
H3G99_RS27020	56541..56774	-	234	WP_000006003.1	DUF905 family protein	-
H3G99_RS27025	56832..57359	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..149181	149181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184520 WP_001312861.1 NZ_CP066518:c52958-52800 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184520 NZ_CP087677:39474-39797 [Xylella taiwanensis]
ATGACTCAACGCGGCGACCTTGTAACGGTTTCCCTGCAAGGCGATTATGGCAAACCTAGACCGGCGTTAATCCTCCAATC
AGACCTGCTGATGGAGCTAGATAGTGTCGTACTGTGTCCGGTCACAAGTGAGCTACGTAATGCGATATTTCGCATCACCG
TTGAACCAAACATAGCCAATGGGCTACGGACGCTCTCGCAAATCATGGTAGACAAAATTTCAACACTGCCGCGTAGCAAA
ATCAGCAAACCTTTTGGACATCTCAATGACGAGAGGATAAAAGCAGTAGAAAAAGCACTGTTGCTCATCGTTGGTATCAT
CTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT184520 NZ_CP066518:c53226-53002 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin