Detailed information of TA system TA184478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2479388..2479905
• Replicon: chromosome
• Accession: NZ_CP066488
• Organism: Staphylococcus aureus strain ncr_155_F133

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: JF379_RS12400
• Protein ID: WP_000113262.1
• Coordinates: 2479388..2479654 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: JF379_RS12405
• Protein ID: WP_000584499.1
• Coordinates: 2479654..2479905 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JF379_RS12375	2474813..2475340	-	528	WP_000973468.1	GNAT family N-acetyltransferase	-
JF379_RS12380	2475584..2476408	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
JF379_RS12385	2476625..2476807	-	183	WP_000230294.1	hypothetical protein	-
JF379_RS12390	2476891..2477358	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
JF379_RS12395	2477544..2479094	-	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
JF379_RS12400	2479388..2479654	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
JF379_RS12405	2479654..2479905	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JF379_RS12410	2480070..2480171	-	102	Protein_2399	hypothetical protein	-
JF379_RS12415	2480178..2480777	-	600	WP_000162810.1	DsbA family protein	-
JF379_RS12420	2480796..2481158	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
JF379_RS12425	2481413..2482663	+	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
JF379_RS12430	2482760..2483491	-	732	WP_000615458.1	amino acid ABC transporter ATP-binding protein	-
JF379_RS12435	2483488..2484207	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T184478 WP_000113262.1 NZ_CP066488:c2479654-2479388 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T184478 NZ_CP087669:350624-350971 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT184478 WP_000584499.1 NZ_CP066488:c2479905-2479654 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT184478 NZ_CP087669:350971-351270 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60