Detailed information of TA system TA184464

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 10671..10876
• Replicon: plasmid p93_3_57kb
• Accession: NZ_CP066482
• Organism: Enterococcus faecium strain Dallas 93_3

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP71_RS14910
• Protein ID: WP_002295624.1
• Coordinates: 10754..10876 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	10671..10772 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP71_RS14880	5876..8080	+	2205	WP_129974412.1	type IA DNA topoisomerase	-
JCP71_RS14885	8096..8245	+	150	WP_002295632.1	hypothetical protein	-
JCP71_RS14890	8319..8912	+	594	WP_002287520.1	transcriptional regulator	-
JCP71_RS14895	8928..9827	+	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP71_RS14900	9830..10315	+	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP71_RS14905	10337..10594	+	258	WP_002305195.1	hypothetical protein	-
-	10671..10772	+	102	NuclAT_0	-	Antitoxin
JCP71_RS14910	10754..10876	-	123	WP_002295624.1	putative holin-like toxin	Toxin
JCP71_RS14915	11030..11290	+	261	WP_049055261.1	hypothetical protein	-
JCP71_RS14920	11733..11933	+	201	WP_002305199.1	hypothetical protein	-
JCP71_RS14925	11938..12375	+	438	WP_002287515.1	hypothetical protein	-
JCP71_RS14930	12368..13075	+	708	WP_002305202.1	hypothetical protein	-
JCP71_RS14935	13456..13635	+	180	WP_002305204.1	hypothetical protein	-
JCP71_RS14940	13817..15112	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57029	57029
	-	flank	IS/Tn	-	-	13817..15112	1295

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184464 WP_002295624.1 NZ_CP066482:c10876-10754 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184464 NZ_CP087663:c2722917-2721595 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 102 bp

>AT184464 NZ_CP066482:10671-10772 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin