Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 127456..127991 Replicon plasmid p93_2_194kb
Accession NZ_CP066474
Organism Enterococcus faecium strain Dallas 93_2

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag JCP72_RS14370 Protein ID WP_002305108.1
Coordinates 127456..127728 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag JCP72_RS14375 Protein ID WP_002303154.1
Coordinates 127728..127991 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP72_RS14340 122462..123649 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
JCP72_RS14345 123837..124784 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
JCP72_RS14350 125081..125629 - 549 WP_002292681.1 hypothetical protein -
JCP72_RS14355 125630..126487 - 858 WP_002292680.1 AAA family ATPase -
JCP72_RS14360 126773..127060 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
JCP72_RS14365 127050..127379 - 330 WP_002292678.1 AbrB family transcriptional regulator -
JCP72_RS14370 127456..127728 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
JCP72_RS14375 127728..127991 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JCP72_RS14380 128097..128513 - 417 WP_002303153.1 hypothetical protein -
JCP72_RS14385 128519..129082 - 564 Protein_136 IS6 family transposase -
JCP72_RS14390 129162..130324 + 1163 WP_086956687.1 IS3 family transposase -
JCP72_RS14395 130796..131695 - 900 WP_002303235.1 aldose 1-epimerase family protein -
JCP72_RS14400 131716..131988 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..194067 194067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T184459 WP_002305108.1 NZ_CP066474:c127728-127456 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T184459 NZ_CP087661:9586-9900 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT184459 WP_002303154.1 NZ_CP066474:c127991-127728 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT184459 NZ_CP087661:9320-9598 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References