Detailed information of TA system TA184453

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 17069..17274
• Replicon: plasmid p91_55kb
• Accession: NZ_CP066467
• Organism: Enterococcus faecium strain Dallas 91

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCQ04_RS14105
• Protein ID: WP_002295624.1
• Coordinates: 17069..17191 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	17173..17274 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCQ04_RS14065	12145..12348	-	204	WP_002300097.1	hypothetical protein	-
JCQ04_RS14070	12508..13083	-	576	WP_002300096.1	recombinase family protein	-
JCQ04_RS14075	13099..13704	-	606	WP_002300095.1	Fic family protein	-
JCQ04_RS14080	14310..14489	-	180	WP_002305204.1	hypothetical protein	-
JCQ04_RS14085	14870..15577	-	708	WP_002305202.1	hypothetical protein	-
JCQ04_RS14090	15570..16007	-	438	WP_002287515.1	hypothetical protein	-
JCQ04_RS14095	16012..16212	-	201	WP_002305199.1	hypothetical protein	-
JCQ04_RS14100	16655..16915	-	261	WP_049055261.1	hypothetical protein	-
JCQ04_RS14105	17069..17191	+	123	WP_002295624.1	putative holin-like toxin	Toxin
-	17173..17274	-	102	NuclAT_0	-	Antitoxin
JCQ04_RS14110	17351..17608	-	258	WP_002305195.1	hypothetical protein	-
JCQ04_RS14115	17630..18115	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCQ04_RS14120	18118..19017	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCQ04_RS14125	19033..19626	-	594	WP_002287520.1	transcriptional regulator	-
JCQ04_RS14130	19700..19849	-	150	WP_002295632.1	hypothetical protein	-
JCQ04_RS14135	19865..22069	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55499	55499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184453 WP_002295624.1 NZ_CP066467:17069-17191 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184453 NZ_CP087657:c5430418-5430035 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184453 NZ_CP066467:c17274-17173 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin